Journal article
Identification and cloning of localized maternal RNAs from xenopus eggs
Cell (Cambridge), Vol.42(3), pp.769-777
1985
DOI: 10.1016/0092-8674(85)90273-9
PMID: 2414011
Abstract
A central question in developmental biology is to explain how cells in different regions of an embryo acquire different developmental fates. We have begun to address this question by investigating whether specific RNAs are localized within a frog egg. Differential screening of a cDNA library shows that most maternal RNAs are uniformly distributed along the animal-vegetal axis. However, we find that a rare class of maternal RNAs is localized. cDNA clones of four localized RNAs have been characterized. Three of these cDNAs are derived from maternal RNAs that are concentrated in the animal hemisphere of unfertilized eggs and remain localized through the early blastula stage. One cDNA is derived from a maternal RNA found almost exclusively in the vegetal hemisphere at both stages. These studies show that some informational molecules, specifically RNAs, are localized in eggs and are inherited by particular blastomeres.
Details
- Title: Subtitle
- Identification and cloning of localized maternal RNAs from xenopus eggs
- Creators
- M. R REBAGLIATI - Harvard univ., dep. biochemistry molecular biologyD. L WEEKS - Harvard univ., dep. biochemistry molecular biologyR. P HARVEY - Harvard univ., dep. biochemistry molecular biologyD. A MELTON - Harvard univ., dep. biochemistry molecular biology
- Resource Type
- Journal article
- Publication Details
- Cell (Cambridge), Vol.42(3), pp.769-777
- Publisher
- Cell Press; Cambridge, MA
- DOI
- 10.1016/0092-8674(85)90273-9
- PMID
- 2414011
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Language
- English
- Date published
- 1985
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Biology; Biochemistry and Molecular Biology
- Record Identifier
- 9984025256802771
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