Identification and genetic mapping of a homeobox gene to the 4p16.1 region of human chromosome 4

H S Stadler; B J Padanilam; K Buetow; J C Murray; M Solursh

doi:10.1073/pnas.89.23.11579

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Identification and genetic mapping of a homeobox gene to the 4p16.1 region of human chromosome 4

Journal article

Open access

Peer reviewed

Identification and genetic mapping of a homeobox gene to the 4p16.1 region of human chromosome 4

H S Stadler, B J Padanilam, K Buetow, J C Murray and M Solursh

Proceedings of the National Academy of Sciences - PNAS, Vol.89(23), pp.11579-11583

12/01/1992

DOI: 10.1073/pnas.89.23.11579

PMCID: PMC50596

PMID: 1360670

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Abstract

A human craniofacial cDNA library was screened with a degenerate oligonucleotide probe based on the conserved third helix of homeobox genes. From this screening, we identified a homeobox gene, H6, which shared only 57-65% amino acid identity to previously reported homeodomains. H6 was physically mapped to the 4p16.1 region by using somatic cell hybrids containing specific deletions of human chromosome 4. Linkage data from a single-stranded conformational polymorphism derived from the 3' untranslated region of the H6 cDNA placed this homeobox gene more than 20 centimorgans proximal of the previously mapped HOX7 gene on chromosome 4. Identity comparisons of the H6 homeodomain with previously reported homeodomains reveal the highest identities to be with the Nk class of homeobox genes in Drosophila melanogaster.

Consensus Sequence

Amino Acid Sequence

Humans

Oligodeoxyribonucleotides - chemistry

Molecular Sequence Data

Chromosome Mapping

Chromosomes, Human, Pair 4

Polymorphism, Genetic

Sequence Alignment

Animals

Pedigree

Base Sequence

Chickens

Polymerase Chain Reaction

Mice

Genes, Homeobox

Genetic Linkage

Details

Title: Subtitle: Identification and genetic mapping of a homeobox gene to the 4p16.1 region of human chromosome 4
Creators: H S Stadler - Department of Biology, University of Iowa, Iowa City
B J Padanilam
K Buetow
J C Murray
M Solursh
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.89(23), pp.11579-11583
DOI: 10.1073/pnas.89.23.11579
PMID: 1360670
PMCID: PMC50596
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences; United States
Grant note: HG00355 / NHGRI NIH HHS DE09170 / NIDCR NIH HHS
Language: English
Date published: 12/01/1992
Academic Unit: Anatomy and Cell Biology; Stead Family Department of Pediatrics; Epidemiology; Pediatric Dentistry; Craniofacial Anomalies Research Center; Dental Research
Record Identifier: 9984025699102771

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