Journal article
Identification and in silico structural and functional analysis of a trypsin-like protease from shrimp Macrobrachium carcinus
PeerJ (San Francisco, CA), Vol.8(4), pp.e9030-e9030
04/23/2020
DOI: 10.7717/peerj.9030
PMCID: PMC7183752
PMID: 32351789
Abstract
Macrobrachium carcinus (Linnaeus, 1758) is a species of freshwater shrimp widely distributed from Florida southwards to southern Brazil, including southeast of Mexico. In the present work, we identified a putative trypsin-like protease cDNA fragment of 736 nucleotides from M. carcinus hepatopancreas tissue by the 3′RACE technique and compared the deduced amino acid sequence to other trypsin-related proteases to describe its structure and function relationship. The bioinformatics analyses showed that the deduced amino acid sequence likely corresponds to a trypsin-like protease closely related to brachyurins, which comprise a subset of serine proteases with collagenolytic activity found in crabs and other crustacea. The M. carcinus trypsin-like protease sequence showed a global sequence identity of 94% with an unpublished trypsin from Macrobrachium rosenbergii (GenBank accession no. AMQ98968), and only 57% with Penaeus vannamei trypsin (GenBank accession no. CAA60129). A detailed analysis of the amino acid sequence revealed specific differences with crustacean trypsins, such as the sequence motif at the beginning of the mature protein, activation mechanism of the corresponding zymogen, amino acid residues of the catalytic triad and residues responsible for substrate specificity.
Details
- Title: Subtitle
- Identification and in silico structural and functional analysis of a trypsin-like protease from shrimp Macrobrachium carcinus
- Creators
- José M Viader-Salvadó - Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, MexicoJosé Alberto Aguilar-Briseño - University of Iowa, Microbiology and ImmunologyJuan A Gallegos-López - Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, MexicoJosé A Fuentes-Garibay - Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, MexicoCarlos Alfonso Alvarez-González - Laboratorio de Acuicultura Tropical, División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, MexicoMartha Guerrero-Olazarán - Instituto de Biotecnología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Nuevo León, Mexico
- Resource Type
- Journal article
- Publication Details
- PeerJ (San Francisco, CA), Vol.8(4), pp.e9030-e9030
- DOI
- 10.7717/peerj.9030
- PMID
- 32351789
- PMCID
- PMC7183752
- NLM abbreviation
- PeerJ
- ISSN
- 2167-8359
- eISSN
- 2167-8359
- Language
- English
- Date published
- 04/23/2020
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984130694802771
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