Identification and initial characterization of 6,000 expressed sequenced tags (ESTs) from rat normal-growing cartilage and swarm rat chondrosarcoma cDNA libraries

Jose A Morcuende; Xiao Dong Huang; Jeff Stevens; Tammy A Kucaba; Bart Brown; Hakeem Abdulkawy; Todd E Scheetz; Sergey Malchenko; Fatima Bonaldo; Thomas L Casavant; Bento Soares

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Identification and initial characterization of 6,000 expressed sequenced tags (ESTs) from rat normal-growing cartilage and swarm rat chondrosarcoma cDNA libraries

Journal article

Open access

Peer reviewed

Identification and initial characterization of 6,000 expressed sequenced tags (ESTs) from rat normal-growing cartilage and swarm rat chondrosarcoma cDNA libraries

Jose A Morcuende, Xiao Dong Huang, Jeff Stevens, Tammy A Kucaba, Bart Brown, Hakeem Abdulkawy, Todd E Scheetz, Sergey Malchenko, Fatima Bonaldo, Thomas L Casavant, …

The Iowa orthopaedic journal, Vol.22, pp.28-34

2002

PMCID: PMC1888380

PMID: 12180607

Appears in Diamond Open Access

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1888380/View

Published (Version of record) Open Access

Abstract

Chondrosarcoma is the second most common type of skeletal malignancy with a survival rate at five years for histological grade III of only 29 percent. The development of a reliable chondrosarcoma animal model could enable the study of tumor growth and progression, the effect of the host on tumor behavior, and the effectiveness of various therapeutic modalities. The Swarm rat chondrosarcoma is a tumor tissue line that has been maintained through the years by serial subcutaneous injections, and the histochemical characteristics of the tumor have remained essentially similar in all transplants over the years. This study was designed to initiate the characterization of the Swarm rat chondrosarcoma model by gene expression profiling as compared to normal-growing rat cartilage. Analysis of the gene expression from both libraries revealed a complex pattern of gene expression, including many genes not yet reported to be expressed by chondrocytes. It suggests that the biochemical characterization of growing cartilage and chondrosarcoma reported to date has only begun to describe the complexity of these tissues.

Gene Expression

Animals

Chondrosarcoma - metabolism

Chondrosarcoma - genetics

Rats

Gene Expression Profiling - methods

Tumor Cells, Cultured

Cartilage - metabolism

Rats, Inbred Strains

Disease Models, Animal

Expressed Sequence Tags

Details

Title: Subtitle: Identification and initial characterization of 6,000 expressed sequenced tags (ESTs) from rat normal-growing cartilage and swarm rat chondrosarcoma cDNA libraries
Creators: Jose A Morcuende - Department of Orthopaedic Surgery, University of Iowa, Iowa City 52242, USA. jose-morcuende@uiowa.edu
Xiao Dong Huang
Jeff Stevens
Tammy A Kucaba
Bart Brown
Hakeem Abdulkawy
Todd E Scheetz
Sergey Malchenko
Fatima Bonaldo
Thomas L Casavant
Bento Soares
Resource Type: Journal article
Publication Details: The Iowa orthopaedic journal, Vol.22, pp.28-34
Publisher: Dept. of Orthopaedics, The University of Iowa; United States
PMID: 12180607
PMCID: PMC1888380
ISSN: 1541-5457
eISSN: 1555-1377
Language: English
Date published: 2002
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Stead Family Department of Pediatrics; Orthopedics and Rehabilitation; Ophthalmology and Visual Sciences
Record Identifier: 9983979900602771

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