Journal article
Identification and sequence analysis of a methylase gene in Porphyromonas gingivalis
Nucleic acids research, Vol.19(15), pp.4189-4192
08/11/1991
DOI: 10.1093/nar/19.15.4189
PMCID: PMC328560
PMID: 1870972
Abstract
A gene from the periodontal organism Porphyromonas gingivalis has been identified as encoding a DNA methylase. The gene, referred to as pgiIM, has been sequenced and found to contain a reading frame of 864 basepairs. The putative amino acid sequence of the encoded methylase was 288 amino acids, and shared 47% and 31% homology with the Streptococcus pneumoniae DpnII and E. coli Dam methylases, respectively. The activity and specificity of the pgi methylase (M.PgiI) was confirmed by cloning the gene into a dam- strain of E. coli (JM110) and performing a restriction analysis on the isolated DNA with enzymes whose activities depended upon the methylation state of the DNA. The data indicated that M.PgiI, like DpnII and Dam, methylated the adenine residue within the sequence 5'-GATC-3'.
Details
- Title: Subtitle
- Identification and sequence analysis of a methylase gene in Porphyromonas gingivalis
- Creators
- Jeffrey A. Banas - Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190Joseph J Ferretti - Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190Ann Progulske-Fox - Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190
- Resource Type
- Journal article
- Publication Details
- Nucleic acids research, Vol.19(15), pp.4189-4192
- DOI
- 10.1093/nar/19.15.4189
- PMID
- 1870972
- PMCID
- PMC328560
- ISSN
- 0305-1048
- eISSN
- 1362-4962
- Language
- English
- Date published
- 08/11/1991
- Academic Unit
- Pediatric Dentistry; Dental Research
- Record Identifier
- 9984066079202771
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