Journal article
Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
Scientific reports, Vol.11(1), pp.22113-22113
11/11/2021
DOI: 10.1038/s41598-021-01701-9
PMCID: PMC8586351
PMID: 34764420
Abstract
In our previous research showed that tramadol having potential anti-tumor effect was associated with enhancement of oncological prognosis in patients with breast cancer surgery. As these effects have not been confirmed by clinical dose-regulated animal or prospective human studies, we investigated the anti-tumor effect of tramadol in vivo. Female nude mice orthotopically inoculated with luciferase-expressing MCF-7 cells, were randomly divided into the control (saline), tramadol group 1 (1.5 mg kg(-1) day(-1)), tramadol group 2 (3 mg kg(-1) day(-1)), and morphine (0.5 mg kg(-1) day(-1)) (n = 5/group). Bioluminescence signals after D-luciferin injection, tumor size, and tumor weight were compared among groups after 4 weeks. Estrogen receptor (ER), progesterone receptor (PR), and transient receptor potential vanilloid (TRPV)-1 expression, natural killer (NK) cell activity, and serum interleukin (IL)-1 beta, tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and interleukin (IL)-6 were then examined. Tumour growth was attenuated in tramadol-treated groups (P < 0.05). NK cell activity was significantly decreased only in the morphine treated group not in sham, control, and tramadol groups. The expression levels of ER alpha, PR alpha and beta, and TRPV1 were decreased in tramadol group 2 compared with those in the morphine group, but not compared to the control group. Serum levels of IL-6 and TNF alpha were reduced in both tramadol-treated group 1 and 2 compared to the control group. Overall, clinical dose of tramadol has anti-tumour effects on MCF-7 cell-derived breast cancer in a xenograft mouse model.
Details
- Title: Subtitle
- Identification for antitumor effects of tramadol in a xenograft mouse model using orthotopic breast cancer cells
- Creators
- Myoung Hwa Kim - Yonsei UniversityJeong-Rim Lee - Yonsei UniversityKi-Joon Kim - Yonsei UniversityJi Hae Jun - Yonsei UniversityHye Jeong Hwang - Yonsei UniversityWootaek Lee - Yonsei UniversitySeung Hyun Nam - Yonsei UniversityJu Eun Oh - Yonsei UniversityYoung Chul Yoo - Yonsei University
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.11(1), pp.22113-22113
- DOI
- 10.1038/s41598-021-01701-9
- PMID
- 34764420
- PMCID
- PMC8586351
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Publisher
- NATURE PORTFOLIO
- Number of pages
- 8
- Grant note
- 2019-32-0023 / Yonsei University College of Medicine NRF-2017R1D1A1B03034659 / National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning; National Research Foundation of Korea; Ministry of Science, ICT & Future Planning, Republic of Korea
- Language
- English
- Date published
- 11/11/2021
- Academic Unit
- Anesthesia
- Record Identifier
- 9984806613002771
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