Identification of Novel Respiratory Syncytial Virus CD4 + and CD8 + T Cell Epitopes in C57BL/6 Mice

Megan E Schmidt; Steven M Varga

doi:10.4049/immunohorizons.1800056

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Identification of Novel Respiratory Syncytial Virus CD4 + and CD8 + T Cell Epitopes in C57BL/6 Mice

Journal article

Open access

Peer reviewed

Identification of Novel Respiratory Syncytial Virus CD4 + and CD8 + T Cell Epitopes in C57BL/6 Mice

Megan E Schmidt and Steven M Varga

ImmunoHorizons, Vol.3(1), pp.1-12

01/14/2019

DOI: 10.4049/immunohorizons.1800056

PMCID: PMC7316092

PMID: 31356172

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https://doi.org/10.4049/immunohorizons.1800056View

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Abstract

Respiratory syncytial virus (RSV) is the most common cause of lower respiratory tract infection and hospitalization in infants. It is well established that both CD4 and CD8 T cells are critical for mediating viral clearance but also contribute to the induction of immunopathology following RSV infection. C57BL/6 mice are often used to study T cell responses following RSV infection given the wide variety of genetically modified animals available. To date, few RSV-derived CD4 and CD8 T cell epitopes have been identified in C57BL/6 mice. Using an overlapping peptide library spanning the entire RSV proteome, intracellular cytokine staining for IFN-γ was performed to identify novel CD4 and CD8 T cell epitopes in C57BL/6 mice. We identified two novel CD4 T cell epitopes and three novel CD8 T cell epitopes located within multiple RSV proteins. Additionally, we characterized the newly described T cell epitopes by determining their TCR Vβ expression profiles and MHC restriction. Overall, the novel RSV-derived CD4 and CD8 T cell epitopes identified in C57BL/6 mice will aid in future studies of RSV-specific T cell responses.

Animals

CD4-Positive T-Lymphocytes - immunology

CD8-Positive T-Lymphocytes - immunology

Cell Line, Tumor

Epitopes, T-Lymphocyte - metabolism

Female

Histocompatibility Antigens Class I - immunology

Interferon-gamma - metabolism

Lung - pathology

Mice

Mice, Inbred C57BL

Peptide Library

Receptors, Antigen, T-Cell, alpha-beta - metabolism

Respiratory Syncytial Virus Infections - pathology

Respiratory Syncytial Virus, Human - metabolism

Viral Proteins - metabolism

Details

Title: Subtitle: Identification of Novel Respiratory Syncytial Virus CD4 + and CD8 + T Cell Epitopes in C57BL/6 Mice
Creators: Megan E Schmidt - University of Iowa
Steven M Varga - University of Iowa
Resource Type: Journal article
Publication Details: ImmunoHorizons, Vol.3(1), pp.1-12
DOI: 10.4049/immunohorizons.1800056
PMID: 31356172
PMCID: PMC7316092
NLM abbreviation: Immunohorizons
ISSN: 2573-7732
eISSN: 2573-7732
Grant note: T32 AI007485 / National Institute of Allergy and Infectious Diseases Extramural Activities R01 AI124093 / National Institute of Allergy and Infectious Diseases Extramural Activities S10 OD016199 / Office of the Director
Language: English
Date published: 01/14/2019
Academic Unit: Graduate College Admin and Gen; Microbiology and Immunology; Pathology; Community and Behavioral Health
Record Identifier: 9984297324202771

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