Journal article
Identification of Novel lncRNAs in Ovarian Cancer and Their Impact on Overall Survival
International journal of molecular sciences, Vol.22(3), pp.1-13
02/01/2021
DOI: 10.3390/ijms22031079
PMCID: PMC7865736
PMID: 33499129
Abstract
Long non-coding RNA's (lncRNA) are RNA sequences that do not encode proteins and are greater than 200 nucleotides in length. They regulate complex cellular mechanisms and have been associated with prognosis in various types of cancer. We aimed to identify lncRNA sequences that are associated with high grade serous ovarian cancer (HGSC) and assess their impact on overall survival. RNA was extracted from 112 HGSC patients and 12 normal fallopian tube samples from our Biobank tissue repository. RNA was sequenced and the Ultrafast and Comprehensive lncRNA detection and quantification pipeline (UClncR) was used for the identification of lncRNA sequences. Univariate logistic and multivariate lasso regression analyses identified lncRNA that was associated with HGSC. Univariate and multivariate Cox proportional hazard ratios were used to evaluate independent predictors of survival. 1943 of 16,325 investigated lncRNA's were differentially expressed in HGSC as compared to controls (p < 0.001). Nine of these demonstrated association with cancer after multivariate lasso regression. Our multivariate analysis of survival identified four lncRNA's associated with survival in HGSC. Three out of these four were found to be independently significant after accounting for all clinical covariates. Lastly, seven lncRNAs were independently associated with initial response to chemotherapy; four portended a worse response, while three were associated with improved response. More research is needed, but there is potential for these lncRNAs to be used as biomarkers of HGSC or predictors of treatment outcome in the future.
Details
- Title: Subtitle
- Identification of Novel lncRNAs in Ovarian Cancer and Their Impact on Overall Survival
- Creators
- Nicholas Cardillo - University of Iowa Hospitals and ClinicsDouglas Russo - University of Iowa Hospitals and ClinicsAndreea Newtson - University of Iowa Hospitals and ClinicsHenry Reyes - University at Buffalo, State University of New YorkYasmin Lyons - The University of Texas at San AntonioEric Devor - University of Iowa Hospitals and ClinicsDavid Bender - University of Iowa Hospitals and ClinicsMichael J. Goodheart - University of Iowa Hospitals and ClinicsJesus Gonzalez-Bosquet - University of Iowa Hospitals and Clinics
- Resource Type
- Journal article
- Publication Details
- International journal of molecular sciences, Vol.22(3), pp.1-13
- DOI
- 10.3390/ijms22031079
- PMID
- 33499129
- PMCID
- PMC7865736
- NLM abbreviation
- Int J Mol Sci
- ISSN
- 1661-6596
- eISSN
- 1422-0067
- Publisher
- Mdpi
- Number of pages
- 13
- Grant note
- American Association of Obstetricians and Gynecologists Foundation (AAOGF) Bridge Funding Award P30CA086862 / NIH/NCI; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) R01 CA99908; R01 CA184101 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Department of Obstetrics & Gynecology at the University of Iowa
- Language
- English
- Date published
- 02/01/2021
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9984318223202771
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