Journal article
Identification of PDGFR as a receptor for AAV-5 transduction
Nature medicine, Vol.9(10), pp.1306-1312
10/01/2003
DOI: 10.1038/nm929
PMID: 14502277
Abstract
Understanding the process of vector transduction has important implications for the application and optimal use of a vector system for human gene therapy. Recent studies with vectors based on adeno-associated virus type 5 (AAV-5) have shown utility of this vector system in the lung, central nervous system, muscle and eye. To understand the natural tropism of this virus and to identify proteins necessary for AAV-5 transduction, we characterized 43 cell lines as permissive or nonpermissive for AAV-5 transduction and compared the gene expression profiles derived from cDNA microarray analyses of those cell lines. A statistically significant correlation was observed between expression of the platelet-derived growth factor receptor (PDGFR-α-polypeptide) and AAV-5 transduction. Subsequent experiments confirmed the role of PDGFR-α and PDGFR-β as receptors for AAV-5. The tropism of AAV-5 in vivo also correlated with the expression pattern of PDGFR-α.
Details
- Title: Subtitle
- Identification of PDGFR as a receptor for AAV-5 transduction
- Creators
- John A Chiorini - National Institute of Dental and Craniofacial ResearchGiovanni Di Pasquale - National Institute of Dental and Craniofacial ResearchBeverly L Davidson - University of IowaColleen S Stein - University of IowaInês Martins - University of IowaDominic Scudiero - Science Applications International CorporationAnne Monks - Science Applications International Corporation
- Resource Type
- Journal article
- Publication Details
- Nature medicine, Vol.9(10), pp.1306-1312
- DOI
- 10.1038/nm929
- PMID
- 14502277
- ISSN
- 1078-8956
- eISSN
- 1546-170X
- Language
- English
- Date published
- 10/01/2003
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984618508702771
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