Identification of RNA aptamers that internalize into HPV-16 E6/E7 transformed tonsillar epithelial cells

Francoise A Gourronc; William M Rockey; William H Thiel; Paloma H Giangrande; Aloysius J Klingelhutz

doi:10.1016/j.virol.2013.08.015

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Identification of RNA aptamers that internalize into HPV-16 E6/E7 transformed tonsillar epithelial cells

Journal article

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Identification of RNA aptamers that internalize into HPV-16 E6/E7 transformed tonsillar epithelial cells

Francoise A Gourronc, William M Rockey, William H Thiel, Paloma H Giangrande and Aloysius J Klingelhutz

Virology (New York, N.Y.), Vol.446(1-2), pp.325-333

11/2013

DOI: 10.1016/j.virol.2013.08.015

PMCID: PMC3812236

PMID: 24074596

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Abstract

Human papillomavirus type 16 (HPV-16) associated oropharyngeal cancers are on a significant increase and better therapeutic strategies are needed. The HPV-16 oncogenes E6 and E7 are expressed in HPV-associated cancers and are able to transform human tonsillar epithelial cells (HTECs). We used cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) to select for RNA aptamers that entered into HPV-16 E6/E7-HTECs. After 12 rounds of cell-SELEX, a pool of aptamers was obtained that had significantly greater internalization capacity (~5-fold) into E6/E7-HTECs as compared to primary HTECs or fibroblasts. Analysis of individual aptamers from the pool indicated variable internalization into E6/E7-HTECs (1–8-fold as compared to a negative control). Most of the individual aptamers internalized into E6/E7 and primary HTECs with similar efficiency, while one aptamer exhibited ~3-fold better internalization into E6/E7-HTECs. Aptamers that internalize into cells may be useful for delivering therapeutic agents to HPV-16 associated malignancies. •Experience with cell-SELEX is to isolate internalizing RNA aptamers is described.•Aptamers that internalize into HPV-16 transformed tonsillar epithelial cells are identified.•Identifying aptamers with high specificity for transformed cells is problematic.•Aptamers may be useful for delivering therapeutic agents to cells.

Papillomavirus

Oropharyngeal

Head neck

Aptamers

SELEX

HPV

Details

Title: Subtitle: Identification of RNA aptamers that internalize into HPV-16 E6/E7 transformed tonsillar epithelial cells
Creators: Francoise A Gourronc - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
William M Rockey - Department of Radiation Oncology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
William H Thiel - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Paloma H Giangrande - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Aloysius J Klingelhutz - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.446(1-2), pp.325-333
DOI: 10.1016/j.virol.2013.08.015
PMID: 24074596
PMCID: PMC3812236
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Grant note: name: NIH, award: R21DEO19953
Language: English
Date published: 11/2013
Academic Unit: Microbiology and Immunology; Radiation Oncology; Internal Medicine
Record Identifier: 9984001208102771

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