Identification of a CD4+ T cell epitope within the M protein of a neurotropic coronavirus

Shurong Xue; Andrew Jaszewski; Stanley Perlman

doi:10.1006/viro.1995.1140

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Identification of a CD4+ T cell epitope within the M protein of a neurotropic coronavirus

Journal article

Open access

Peer reviewed

Identification of a CD4+ T cell epitope within the M protein of a neurotropic coronavirus

Shurong Xue, Andrew Jaszewski and Stanley Perlman

Virology, Vol.208(1), pp.173-179

04/01/1995

DOI: 10.1006/viro.1995.1140

PMID: 11831697

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Abstract

A significant CD4+ T cell response against the transmembrane (M) protein can be detected in the spleens of C57Bl/6 mice infected intraperitoneally with a sublethal injection of the neurotropic JHM strain of mouse hepatitis virus (MHV-JHM), but not in those of mice with the chronic demyelinating encephalomyelitis caused by this virus. With the ultimate goal of determining the role of the M-specific response in the pathogenesis of MHV-JHM-induced neurological diseases, CD4+ T cell epitopes within the M protein were identified using vaccinia virus recombinants expressing truncated forms of the protein and peptides spanning most of the M protein in cell proliferation assays. Peptides covering residues 128-147 contain at least one CD4+ T cell epitope for MHV-JHM. Within this region is a sequence (residues 135-143) which matches the recently described MHC class II I-Ab binding motif. Delineation of this epitope should facilitate analysis of the role of the M-specific CD4+ T cell response in the development of acute and chronic neurological infections caused by MHV-JHM.

Immunodominant Epitopes - immunology

Viral Matrix Proteins - genetics

Mice, Inbred C57BL

Epitopes, T-Lymphocyte - genetics

Recombinant Proteins - genetics

CD4-Positive T-Lymphocytes - immunology

Murine hepatitis virus - immunology

Animals

Recombinant Proteins - immunology

Mice

Viral Matrix Proteins - immunology

Epitopes, T-Lymphocyte - immunology

Immunodominant Epitopes - genetics

Tumor Cells, Cultured

Nervous System - virology

Murine hepatitis virus - genetics

Details

Title: Subtitle: Identification of a CD4+ T cell epitope within the M protein of a neurotropic coronavirus
Creators: Shurong Xue - Department of Microbiology, University of Iowa, Iowa City 52242, USA
Andrew Jaszewski
Stanley Perlman
Resource Type: Journal article
Publication Details: Virology, Vol.208(1), pp.173-179
DOI: 10.1006/viro.1995.1140
PMID: 11831697
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: United States
Grant note: NS24401 / NINDS NIH HHS
Language: English
Date published: 04/01/1995
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777478302771

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