Journal article
Identification of a Gene (GPR30) with Homology to the G-Protein-Coupled Receptor Superfamily Associated with Estrogen Receptor Expression in Breast Cancer
Genomics (San Diego, Calif.), Vol.45(3), pp.607-617
11/01/1997
DOI: 10.1006/geno.1997.4972
PMID: 9367686
Abstract
Using the technique of differential cDNA library screening, a cDNA clone was isolated from an estrogen receptor (ER)-positive breast carcinoma cell line (MCF7) cDNA library based upon the overexpression of this gene compared to an ER-negative cell line (MDA-MB-231). Sequence analysis of this clone determined that it shared significant homology to G-protein-coupled receptors. This receptor, GPCR-Br, was abundantly expressed in the ER-positive breast carcinoma cell lines MCF7, T-47D, and MDA-MB-361. Expression was absent or minimal in the ER-negative breast carcinoma cell lines BT-20, MDA-MB-231, and HBL-100. GPCR-Br was ubiquitously expressed in human tissues examined but was most abundant in placenta. GPCR-Br expression was examined in 11 primary breast carcinomas. GPCR-Br was detected in all 4 ER-positive tumors and only 1 of 7 ER-negative tumors. Based upon PCR analysis in hybrid cell lines, the gene for GPCR-Br (HGMW-approved symbol GPR30) was mapped to chromosome 7p22. The pattern of expression of GPCR-Br indicates that this receptor may be involved in physiologic responses specific to hormonally responsive tissues.
Details
- Title: Subtitle
- Identification of a Gene (GPR30) with Homology to the G-Protein-Coupled Receptor Superfamily Associated with Estrogen Receptor Expression in Breast Cancer
- Creators
- Charles Carmeci - Department of Surgery, Stanford University, Stanford, California, 94305Devon A Thompson - Department of Surgery, Stanford University, Stanford, California, 94305Huijun Z Ring - Department of Genetics, Stanford University, Stanford, California, 94305Uta Francke - Department of Genetics, Stanford University, Stanford, California, 94305Ronald J Weigel - Department of Surgery, Stanford University, Stanford, California, 94305
- Resource Type
- Journal article
- Publication Details
- Genomics (San Diego, Calif.), Vol.45(3), pp.607-617
- DOI
- 10.1006/geno.1997.4972
- PMID
- 9367686
- NLM abbreviation
- Genomics
- ISSN
- 0888-7543
- eISSN
- 1089-8646
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 11/01/1997
- Academic Unit
- Molecular Physiology and Biophysics; Anatomy and Cell Biology; Surgery; Biochemistry and Molecular Biology
- Record Identifier
- 9984025250802771
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