Journal article
Identification of a Mammalian Golgi Sec1p-like Protein, mVps45
The Journal of biological chemistry, Vol.272(10), pp.6187-6193
03/07/1997
DOI: 10.1074/jbc.272.10.6187
PMID: 9045632
Abstract
Our understanding of lysosomal biogenesis and general vesicular transport in animal cells has been greatly enhanced by studies of vacuolar biogenesis in yeast. Genetic screens have identified a number of proteins that play direct roles in the proper sorting of vacuolar hydrolases. These include t-SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins and Sec1p-like proteins, which have recently been implicated as key regulators of vesicle fusion. In this study we have extended these observations in yeast and have isolated and characterized a novel member of the Sec1p-like family of proteins from mammalian cells, mVps45. mVps45 shares a high level of identity with the Saccharomyces cerevisiae Sec1p-like protein Vps45p that is believed to function with the t-SNARE Pep12p in the fusion of Golgi-derived transport vesicles with a prevacuolar compartment. We found that mVps45 is a ubiquitously expressed peripheral membrane protein that localized to perinuclear Golgi-like and trans-Golgi network compartments in Chinese hamster overy cells. We found that mVps45 could bind specifically to yeast Pep12p and to the mammalian Pep12p-like protein, syntaxin 6, in vitro
Details
- Title: Subtitle
- Identification of a Mammalian Golgi Sec1p-like Protein, mVps45
- Creators
- Judy T. Tellam - University of QueenslandDavid E. James - University of QueenslandTom H. Stevens - University of OregonRobert C. Piper - University of Oregon
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.272(10), pp.6187-6193
- DOI
- 10.1074/jbc.272.10.6187
- PMID
- 9045632
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- Elsevier Inc
- Grant note
- Dora Lush Postgraduate Fellowship (to J. T. T.) GM16601-01 / Postdoctoral Fellowship National Institutes of Health Wellcome Trust and the National Health and Medical Research Council (to D. E. J.) GM32448 (to T. H. S.) / National Institutes of Health (to R. C. P.)
- Language
- English
- Date published
- 03/07/1997
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297613702771
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