Identification of a Novel Protein Kinase A Anchoring Protein That Binds Both Type I and Type II Regulatory Subunits

Lily Jun-shen Huang; Kyle Durick; Joshua A Weiner; Jerold Chun; Susan S Taylor

doi:10.1074/jbc.272.12.8057

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Identification of a Novel Protein Kinase A Anchoring Protein That Binds Both Type I and Type II Regulatory Subunits

Journal article

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Identification of a Novel Protein Kinase A Anchoring Protein That Binds Both Type I and Type II Regulatory Subunits

Lily Jun-shen Huang, Kyle Durick, Joshua A Weiner, Jerold Chun and Susan S Taylor

The Journal of biological chemistry, Vol.272(12), pp.8057-8064

03/21/1997

DOI: 10.1074/jbc.272.12.8057

PMID: 9065479

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Abstract

Compartmentalization of cAMP-dependent protein kinase is achieved in part by interaction with A-kinase anchoring proteins (AKAPs). All of the anchoring proteins identified previously target the kinase by tethering the type II regulatory subunit. Here we report the cloning and characterization of a novel anchoring protein, D-AKAP1, that interacts with the N terminus of both type I and type II regulatory subunits. A novel cDNA encoding a 125-amino acid fragment of D-AKAP1 was isolated from a two-hybrid screen and shown to interact specifically with the type I regulatory subunit. Although a single message of 3.8 kilobase pairs was detected for D-AKAP1 in all embryonic stages and in most adult tissues, cDNA cloning revealed the possibility of at least four splice variants. All four isoforms contain a core of 526 amino acids, which includes the R binding fragment, and may be expressed in a tissue-specific manner. This core sequence was homologous to S-AKAP84, including a mitochondrial signal sequence near the amino terminus (Lin, R. Y., Moss, S. B., and Rubin, C. S. (1995) J. Biol. Chem. 270, 27804-27811). D-AKAP1 and the type I regulatory subunit appeared to have overlapping expression patterns in muscle and olfactory epithelium by in situ hybridization. These results raise a novel possibility that the type I regulatory subunit may be anchored via anchoring proteins.

Details

Title: Subtitle: Identification of a Novel Protein Kinase A Anchoring Protein That Binds Both Type I and Type II Regulatory Subunits
Creators: Lily Jun-shen Huang
Kyle Durick
Joshua A Weiner
Jerold Chun
Susan S Taylor
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.272(12), pp.8057-8064
DOI: 10.1074/jbc.272.12.8057
PMID: 9065479
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Language: English
Date published: 03/21/1997
Academic Unit: Liberal Arts and Science Admin; Psychiatry; Iowa Neuroscience Institute; Biology
Record Identifier: 9983997990102771

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