Logo image
Back
Identification of a transferable two-amino-acid motif (GT) present in the C-terminal tail of the human lutropin receptor that redirects internalized G protein-coupled receptors from a degradation to a recycling pathway
Journal article   Open access   Peer reviewed

Identification of a transferable two-amino-acid motif (GT) present in the C-terminal tail of the human lutropin receptor that redirects internalized G protein-coupled receptors from a degradation to a recycling pathway

Colette Galet, Le Min, Ramesh Narayanan, Mikiko Kishi, Nancy L Weigel and Mario Ascoli
Molecular endocrinology (Baltimore, Md.), Vol.17(3), pp.411-422
03/2003
DOI: 10.1210/me.2002-0161
PMID: 12554787
url
https://doi.org/10.1210/me.2002-0161View
Published (Version of record) Open Access

Abstract

Although highly homologous in amino acid sequence, the agonist-receptor complexes formed by the human lutropin receptor (hLHR) and rat (r) LHR follow different intracellular routes. The agonist-rLHR complex is routed mostly to a lysosomal degradation pathway whereas a substantial portion of the agonist-hLHR complex is routed to a recycling pathway. In a previous study, we showed that grafting a five-residue sequence (GTALL) present in the C-terminal tail of the hLHR into the equivalent position of the rLHR redirects a substantial portion of the internalized agonist-rLHR complex to a recycling pathway. Using a number of mutations of the GTALL motif, we now show that only the first two residues (GT) of this motif are necessary and sufficient to induce recycling of the internalized agonist-rLHR complex. Phosphoamino acid analysis and mutations of the GT motif show that phosphorylation of the threonine residue is not necessary for recycling. Lastly, we show that addition of portions of the C-terminal tail of the hLHR that include the GT motif to the C-terminal tails of the rat follitropin or murine delta-opioid receptors promotes the post-endocytotic recycling of these G protein-coupled receptors.We conclude that the GT motif present in the C-terminal tail of the hLHR is a transferable motif that promotes the postendocytotic recycling of several G protein-coupled receptors and that the GT-induced recycling does not require the phosphorylation of the threonine residue.
 Amino Acid Motifs - physiology Amino Acid Sequence Animals Cathepsin D - metabolism Cells, Cultured Chorionic Gonadotropin - metabolism Down-Regulation - physiology Electrophoresis, Gel, Two-Dimensional Endocytosis - physiology Follicle Stimulating Hormone - metabolism GTP-Binding Proteins - metabolism Humans Microscopy, Confocal Molecular Sequence Data Phosphoamino Acids - analysis Precipitin Tests rab5 GTP-Binding Proteins - metabolism Rats Receptors, LH - genetics Receptors, LH - metabolism Receptors, Opioid, delta - metabolism Sequence Alignment

Details

Metrics

1 Record Views
Logo image