Identification of androgen-coregulated protein networks from the microsomes of human prostate cancer cells

Michael Wright; Jimmy Eng; James Sherman; David Hockenbery; Peter Nelson; Timothy Galitski; Ruedi Aebersold

doi:10.1186/gb-2003-5-1-r4

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Identification of androgen-coregulated protein networks from the microsomes of human prostate cancer cells

Journal article

Open access

Identification of androgen-coregulated protein networks from the microsomes of human prostate cancer cells

Michael Wright, Jimmy Eng, James Sherman, David Hockenbery, Peter Nelson, Timothy Galitski and Ruedi Aebersold

GenomeBiology.com, Vol.5(1), pp.R4-R4

2003

DOI: 10.1186/gb-2003-5-1-r4

PMID: 14709176

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Abstract

BACKGROUND: Androgens play a critical role in the development of prostate cancer-dysregulation of androgen-regulated growth pathways can led to hormone-refractory prostate cancer. A comprehensive understanding of androgen-regulated cellular processes has not been achieved to date. To this end, we have applied a large-scale proteomic approach to define cellular processes that are responsive to androgen treatment in LNCaP prostate cancer cells. RESULTS: Using isotope-coded affinity tags and mass spectrometry we identified and quantified the relative abundance levels of 1,064 proteins and found that distinct cellular processes were coregulated by androgen while others were essentially unaffected. Subsequent pharmacological perturbation of the cellular process for energy generation confirmed that androgen starvation had a profound effect on this pathway. CONCLUSIONS: Our results provide evidence for the role of androgenic hormones in coordinating the expression of critical components involved in distinct cellular processes and further establish a foundation for the comprehensive reconstruction of androgen-regulated protein networks and pathways in prostate cancer cells.

Details

Title: Subtitle: Identification of androgen-coregulated protein networks from the microsomes of human prostate cancer cells
Creators: Michael Wright
Jimmy Eng
James Sherman
David Hockenbery
Peter Nelson
Timothy Galitski
Ruedi Aebersold
Resource Type: Journal article
Publication Details: GenomeBiology.com, Vol.5(1), pp.R4-R4
DOI: 10.1186/gb-2003-5-1-r4
PMID: 14709176
NLM abbreviation: Genome Biol
ISSN: 1465-6906
eISSN: 1465-6914
Publisher: BioMed Central Ltd
Language: English
Date published: 2003
Academic Unit: Molecular Physiology and Biophysics
Record Identifier: 9984025444602771

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