Identification of cytokeratins as accessory mediators of Salmonella entry into eukaryotic cells

Steve A Carlson; M Bishr Omary; Bradley D Jones

doi:10.1016/S0024-3205(01)01512-0

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Identification of cytokeratins as accessory mediators of Salmonella entry into eukaryotic cells

Journal article

Peer reviewed

Identification of cytokeratins as accessory mediators of Salmonella entry into eukaryotic cells

Steve A Carlson, M Bishr Omary and Bradley D Jones

Life sciences (1973), Vol.70(12), pp.1415-1426

02/08/2002

DOI: 10.1016/S0024-3205(01)01512-0

PMID: 11883717

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Abstract

Pathogenic Salmonella species initiate infection of a mammalian host by inducing their own uptake into intestinal M-cells. During the uptake process, the bacteria utilize an intrinsic secretion system to release proteins that enter host cells. The secreted invasion-mediating proteins subsequently interact with host cell components that induce alterations in the actin cytoskeleton. To identify potential cellular determinants of invasion, we employed a yeast two-hybrid system using the secreted Salmonella invasion protein (SipC) as the bait protein. This system identified cytokeratins, supportive components of the cytoskeletal matrix, as proteins that may physically interact with SipC. Transfection-based studies revealed an inhibition of Salmonella invasion when a dominant negative cytokeratin-18 was expressed. Immunofluorescent confocal microscopy studies revealed that Salmonella did not enter HEp-2 cells expressing the dominant negative cytokeratin-18. These results suggest that an interaction between SipC and cytokeratin-18 may occur as part of Salmonella invasion.

Humans

Actins - ultrastructure

Molecular Sequence Data

Eukaryotic Cells - metabolism

Saccharomyces cerevisiae - metabolism

Keratins - metabolism

Transfection

Base Sequence

Salmonella typhimurium - classification

Keratins - ultrastructure

Membrane Proteins - metabolism

Tumor Cells, Cultured

Eukaryotic Cells - microbiology

Amino Acid Sequence

Salmonella typhimurium - physiology

Salmonella typhimurium - pathogenicity

Membrane Proteins - genetics

Bacterial Proteins - genetics

Transcription Factors - metabolism

Microscopy, Confocal

Virulence - physiology

Bacterial Proteins - metabolism

Salmonella Infections - microbiology

Keratins - genetics

Fungal Proteins - metabolism

Details

Title: Subtitle: Identification of cytokeratins as accessory mediators of Salmonella entry into eukaryotic cells
Creators: Steve A Carlson - Preharvest Food Safety and Enteric Disease Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA. scarlson@nadc.ars.usda.gov
M Bishr Omary
Bradley D Jones
Resource Type: Journal article
Publication Details: Life sciences (1973), Vol.70(12), pp.1415-1426
DOI: 10.1016/S0024-3205(01)01512-0
PMID: 11883717
ISSN: 0024-3205
eISSN: 1879-0631
Grant note: DK 07663 / NIDDK NIH HHS A138268 / PHS HHS
Language: English
Date published: 02/08/2002
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083820702771

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