Journal article
Identification of dehydration responsive genes from two non-nodulated alfalfa cultivars using Medicago truncatula microarrays
Acta physiologiae plantarum, Vol.30(2), pp.183-199
03/2008
DOI: 10.1007/s11738-007-0107-5
Abstract
To have a comprehensive understanding of how legume plants respond to drought at the gene expression level and examine whether legume plants that are not fixing nitrogen would behave similar to non-legume plants in drought response, transcriptomes were studied in two non-nodulated alfalfa (Medicago sativa L.) cultivars, Ladak and 53V08, when plants were subjected to dehydration stress. Two heat shock-related protein genes were up-regulated in the 3-h stressed shoots in both cultivars. One of them was also up-regulated in the 8-h stressed shoots, along with dehydrin and LEA. A xyloglucan endotransglycosylase and a gene with unknown function were down-regulated in both 3- and 8-h stressed shoots. In roots, nearly half of the 55 genes commonly up-regulated at 3 h are involved in pathogen resistance, insect defense and flavonoid synthesis, which differs from other dehydration-responsive transcriptomes in the literature. Many known drought-responsive genes, such as LEA and dehydrin, were up-regulated after 8 h of treatment. The genes encoding caffeoyl-CoA O-methyl transferase and dirigent were up-regulated in the 3-h stressed roots, while two aquaporin genes were down-regulated, suggesting that lignification and prevention of water loss in roots in initial dehydration stress is a common strategy for both cultivars. The results also indicate the involvement of some specific signal transduction pathways, osmotic adjustment and ion homeostasis regulation during dehydration response. Besides those known dehydration-responsive genes in the literature, some dehydration responses and genes in alfalfa appear to be unique. Our results provide valuable insight into a comprehensive understanding of dehydration response in alfalfa at the molecular level.
Details
- Title: Subtitle
- Identification of dehydration responsive genes from two non-nodulated alfalfa cultivars using Medicago truncatula microarrays
- Creators
- Dong Chen - Center for Integrated BioSystems Utah State University Logan UT 84322 USAMing-Xiang Liang - Department of Plants, Soils, and Climate Utah State University Logan UT 84322 USADaryll DeWald - Department of Biology Utah State University Logan UT 84322 USABart Weimer - Department of Nutrition and Food Sciences Utah State University Logan UT 84322 USAMichael Peel - USDA-ARS Forage and Range Research Laboratory Utah State University Logan UT 84322 USABruce Bugbee - Department of Plants, Soils, and Climate Utah State University Logan UT 84322 USAJacob Michaelson - Center for Integrated BioSystems Utah State University Logan UT 84322 USAElizabeth Davis - Department of Plants, Soils, and Climate Utah State University Logan UT 84322 USAYajun Wu - Department of Plants, Soils, and Climate Utah State University Logan UT 84322 USA
- Resource Type
- Journal article
- Publication Details
- Acta physiologiae plantarum, Vol.30(2), pp.183-199
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- DOI
- 10.1007/s11738-007-0107-5
- ISSN
- 0137-5881
- eISSN
- 1861-1664
- Language
- English
- Date published
- 03/2008
- Academic Unit
- Communication Sciences and Disorders; Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute
- Record Identifier
- 9984070690802771
Metrics
8 Record Views