Journal article
Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA
Developmental dynamics, Vol.239(6), pp.1838-1848
06/2010
DOI: 10.1002/dvdy.22304
PMCID: PMC3065113
PMID: 20503379
Abstract
RNA localization is a common mechanism for regulating cell structure and function. Localized RNAs in Xenopus oocytes are critical for early development, including germline specification by the germ plasm. Despite the importance of these localized RNAs, only approximately 25 have been identified and fewer are functionally characterized. Using microarrays, we identified a large set of localized RNAs from the vegetal cortex. Overall, our results indicate a minimum of 275 localized RNAs in oocytes, or 2-3% of maternal transcripts, which are in general agreement with previous findings. We further validated vegetal localization for 24 candidates and further characterized three genes expressed in the germ plasm. We identified novel germ plasm expression for reticulon 3.1, exd2 (a novel exonuclease-domain encoding gene), and a putative noncoding RNA. Further analysis of these and other localized RNAs will likely identify new functions of germ plasm and facilitate the identification of cis-acting RNA localization elements.
Details
- Title: Subtitle
- Identification of germ plasm-associated transcripts by microarray analysis of Xenopus vegetal cortex RNA
- Creators
- Tawny N Cuykendall - The University of Iowa, Department of Biology, Iowa City, Iowa 52242-1324, USADouglas W Houston
- Resource Type
- Journal article
- Publication Details
- Developmental dynamics, Vol.239(6), pp.1838-1848
- Publisher
- United States
- DOI
- 10.1002/dvdy.22304
- PMID
- 20503379
- PMCID
- PMC3065113
- ISSN
- 1097-0177
- eISSN
- 1097-0177
- Grant note
- R01 GM083999 / NIGMS NIH HHS 5R01 GM083999-02 / NIGMS NIH HHS R01 GM083999-03 / NIGMS NIH HHS
- Language
- English
- Date published
- 06/2010
- Academic Unit
- Biology
- Record Identifier
- 9983992078402771
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