Journal article
Identification of guinea pig gammadelta T cells and characterization during pulmonary tuberculosis
Veterinary immunology and immunopathology, Vol.102(1-2), pp.33-44
11/2004
DOI: 10.1016/j.vetimm.2004.06.010
PMID: 15451613
Abstract
Guinea pigs are an alternative small animal model for many disease studies. Here we describe a pan-gammadelta monoclonal antibody (anti-TCRdelta1) specific for the constant region of human T cell receptor delta chains that cross-reacts with a subpopulation of guinea pig (Cavia porcellus) lymphocytes. The phenotype and distribution of this subpopulation is consistent with the guinea pig gammadelta T cell subset. FACS analysis of fresh PBMC and splenocytes from naïve guinea pigs revealed the presence of a subset of cells that stained with the anti-TCRdelta1 mAb. The relative percentage of anti-TCRdelta1 positive cells in PBMC and tissues is similar to that described for gammadelta T cells in other species. Immunohistochemistry of tissues also revealed a distribution of anti-TCRdelta1 positive cells consistent with gammadelta T cells. These data are further supported by staining of a polyclonal guinea pig T cell line that became progressively CD4 and CD8 negative in long-term culture. Analysis of PBMC from guinea pigs following aerosol infection with virulent Mycobacterium tuberculosis revealed no apparent changes in the steady-state percentage of blood gammadelta+ T cells. Taken together, these data suggest that the anti-TCRdelta1 antibody recognizes the gammadelta T cell subset in guinea pigs. This reagent may be useful for examining gammadelta T cells in various disease models where the guinea pig is a more desirable model for study.
Details
- Title: Subtitle
- Identification of guinea pig gammadelta T cells and characterization during pulmonary tuberculosis
- Creators
- Xiaowei Xiong - Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Smith 514, 1 Jimmy Fund Way, Boston, MA 02115, USACraig T MoritaJack F BukowskiMichael B BrennerChristopher C Dascher
- Resource Type
- Journal article
- Publication Details
- Veterinary immunology and immunopathology, Vol.102(1-2), pp.33-44
- DOI
- 10.1016/j.vetimm.2004.06.010
- PMID
- 15451613
- NLM abbreviation
- Vet Immunol Immunopathol
- ISSN
- 0165-2427
- eISSN
- 1873-2534
- Grant note
- AI48932 / NIAID NIH HHS AR045504 / NIAMS NIH HHS
- Language
- English
- Date published
- 11/2004
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094498202771
Metrics
16 Record Views