Journal article
Identification of hematopoietic-specific regulatory elements from the CD45 gene and use for lentiviral tracking of transplanted cells
Experimental hematology, Vol.42(9), pp.761-772.e10
09/2014
DOI: 10.1016/j.exphem.2014.05.005
PMCID: PMC4167479
PMID: 24852660
Abstract
The development of a hematopoietic reporter is crucial for determining the fate of lineages derived from cell-based therapies. A marking system will enable safer embryonic stem and induced pluripotent stem cell-based derivation of blood lineages and facilitate the development of efficient cellular reprogramming strategies based on direct fibroblast conversion. Here we report that the protein tyrosine phosphatase CD45 is an ideal candidate gene on which to base a hematopoietic reporter. CD45 regulatory elements were discovered by analyzing transcription factor chromatin occupancy (ChIP-seq) and promoter nuclease sensitivity (DNase-seq) to identify minimally sufficient sequences required for expression. After cloning the CD45 regulatory elements into an attenuated lentiviral backbone, we found that two transcriptional initiation regions were essential for high-level expression. Expressing CD45 promoters containing these regions and tethered to green fluorescent protein (GFP) in a primary B-cell differentiation assay and a transplantation model resulted in high levels of GFP in lymphoid, myeloid, and nucleated erythroid cells in mouse and human blood cell lineages. Moreover, GFP levels remained high 5 months after secondary transplantation, indicating persistence of the reporter. No CD45-driven GFP expression is observed after fibroblast or embryonic stem cell transduction. The GFP reporter is seen only after embryonic stem cells differentiate into hematopoietic cell progenitors and lineages, suggesting that this hematopoietic reporter system could be useful in validating potential autologous blood cell therapies.
Details
- Title: Subtitle
- Identification of hematopoietic-specific regulatory elements from the CD45 gene and use for lentiviral tracking of transplanted cells
- Creators
- Khanh L Duong - Roy J. and Lucille A. Carver College of MedicineSatyabrata Das - Roy J. and Lucille A. Carver College of MedicineShuyang Yu - Roy J. and Lucille A. Carver College of MedicineJennifer Y Barr - Roy J. and Lucille A. Carver College of MedicineSnehalata Jena - Roy J. and Lucille A. Carver College of MedicineEunmi Kim - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa, USANicolas Zavazava - University of IowaJohn D Colgan - University of IowaHai-Hui Xue - University of IowaDana N Levasseur - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Experimental hematology, Vol.42(9), pp.761-772.e10
- DOI
- 10.1016/j.exphem.2014.05.005
- PMID
- 24852660
- PMCID
- PMC4167479
- ISSN
- 0301-472X
- eISSN
- 1873-2399
- Grant note
- R01 AI054821 / NIAID NIH HHS R01 AI093737 / NIAID NIH HHS P30 DK054759 / NIDDK NIH HHS P30 CA086862 / NCI NIH HHS
- Language
- English
- Date published
- 09/2014
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Microbiology and Immunology; Anatomy and Cell Biology; Immunology; Internal Medicine
- Record Identifier
- 9984284339702771
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