Journal article
Identification of host proteins associated with HIV-1 preintegration complexes isolated from infected CD4+ cells
Retrovirology, Vol.7(1), pp.66-66
2010
DOI: 10.1186/1742-4690-7-66
PMCID: PMC2924840
PMID: 20698996
Abstract
An integrated HIV-1 genomic DNA leads to an infected cell becoming either an active or a latent virus-producing cell. Upon appropriate activation, a latently infected cell can result in production of progeny viruses that spread the infection to uninfected cells. The host proteins influence several steps of HIV-1 infection including formation of the preintegration complex (PIC), a key nucleoprotein intermediate essential for integration of reverse transcribed viral DNA into the chromosome. Much effort has gone into the identification of host proteins contributing to the assembly of functional PICs. Experimental approaches included the use of yeast two-hybrid system, co-immunoprecipitation, affinity tagged HIV-1 viral proteins and
in vitro
reconstitution of salt-stripped PIC activity. Several host proteins identified using these approaches have been shown to affect HIV-1 replication in cells and influence catalytic activities of recombinant IN
in vitro
. However, the comprehensive identification and characterization of host proteins associated with HIV-1 PICs of infected cells have been hindered in part by the technical limitation in acquiring sufficient amount of catalytically active PICs. To efficiently identify additional host factors associated with PICs in infected cells, we have developed the following novel approach. The catalytically active PICs from HIV-1-infected CD4
+
cells were isolated using biotinylated target DNA, and the proteins selectively co-purifying with PICs have been analyzed by mass spectrometry. This technology enabled us to reveal at least 19 host proteins that are associated with HIV-1 PICs, of which 18 proteins have not been described previously with respect to HIV-1 integration. Physiological functions of the identified proteins range from chromatin organization to protein transport. A detailed characterization of these host proteins could provide new insights into the mechanism of HIV-1 integration and uncover new antiviral targets to block HIV-1 integration.
Details
- Title: Subtitle
- Identification of host proteins associated with HIV-1 preintegration complexes isolated from infected CD4+ cells
- Creators
- Nidhanapati K Raghavendra - Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, California 91125, USANikolozi Shkriabai - Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, California 91125, USARobert LJ Graham - Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, California 91125, USASonja Hess - Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, California 91125, USAMamuka Kvaratskhelia - Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, California 91125, USALi Wu - Proteome Exploration Laboratory, Beckman Institute, California Institute of Technology, Pasadena, California 91125, USA
- Resource Type
- Journal article
- Publication Details
- Retrovirology, Vol.7(1), pp.66-66
- DOI
- 10.1186/1742-4690-7-66
- PMID
- 20698996
- PMCID
- PMC2924840
- NLM abbreviation
- Retrovirology
- ISSN
- 1742-4690
- eISSN
- 1742-4690
- Publisher
- BioMed Central
- Language
- English
- Date published
- 2010
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001158902771
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