Journal article
Identification of hydroxylated metabolites of 3,3 ',4,4 '-tetrachlorobiphenyl and metabolic pathway in whole poplar plants
Chemosphere, Vol.81(4), pp.523-528
2010
DOI: 10.1016/j.chemosphere.2010.07.036
PMCID: PMC2943055
PMID: 20708213
Abstract
Polychlorinated biphenyls (PCBs) can be metabolized to hydroxylated polychlorinated biphenyls (OH-PCBs) as reported in a number of animal studies. However, there are few studies on OH-PCBs in vivo in whole plants. In order to explore the formation of OH-PCBs in whole plants in detail, poplars (Populus deltoides × nigra, DN34) were exposed to 3,3′,4,4′-tetrachlorobiphenyl (CB77) in hydroponic solution. Poplars are widely used in phytoremediation applications and the complete genome has been sequenced. In this research, a HPLC-MS method was developed to directly determine the hydroxylated metabolites of CB77 (OH-CB77s), avoiding the experimental errors introduced by derivatization pretreatments required by gas chromatography-based methods. Three potential hydroxylated metabolites of CB77, including 6-hydroxy-3,3′,4,4′-tetrachlorobiphenyl (6OH-CB77), 5-hydroxy-3,3′,4,4′-tetrachlorobiphenyl (5OH-CB77) and 4′-hydroxy-3,3′,4,5′-tetrachlorobiphenyl (4′OH-CB79), were determined in poplar tissues. The major product, 6OH-CB77, was detected in the roots, bottom bark, bottom wood, middle bark and middle wood for the whole poplar plants, but the minor product, 5OH-CB77, was detected only in the poplar roots. The concentration of 6OH-CB77 was about 10 times greater than that of 5OH-CB77 in the roots. However, the major mammalian metabolite, 4′OH-CB79 was not detected in any of the samples. The results suggest that the hydroxylated metabolic pathway of CB77 is via an epoxide intermediate in poplar.
Details
- Title: Subtitle
- Identification of hydroxylated metabolites of 3,3 ',4,4 '-tetrachlorobiphenyl and metabolic pathway in whole poplar plants
- Creators
- Guangshu Zhai - University of IowaHans-Joachim Lehmler - University of iowaJerald L. Schnoor - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Chemosphere, Vol.81(4), pp.523-528
- DOI
- 10.1016/j.chemosphere.2010.07.036
- PMID
- 20708213
- PMCID
- PMC2943055
- NLM abbreviation
- Chemosphere
- ISSN
- 0045-6535
- Language
- English
- Date published
- 2010
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health; Iowa Neuroscience Institute; Iowa Superfund Research Program
- Record Identifier
- 9983557792102771
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