Identification of interdependent signals required for anterograde traffic of the ATP-binding cassette transporter protein Yor1p

Eric A Epping; W Scott Moye-Rowley

doi:10.1074/jbc.M202987200

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Identification of interdependent signals required for anterograde traffic of the ATP-binding cassette transporter protein Yor1p

Journal article

Open access

Peer reviewed

Identification of interdependent signals required for anterograde traffic of the ATP-binding cassette transporter protein Yor1p

Eric A Epping and W Scott Moye-Rowley

The Journal of biological chemistry, Vol.277(38), pp.34860-34869

09/20/2002

DOI: 10.1074/jbc.M202987200

PMID: 12107161

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https://doi.org/10.1074/jbc.M202987200View

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Abstract

The plasma membrane ATP-binding cassette (ABC) transporter Yor1p mediates oligomycin resistance in Saccharomyces cerevisiae. Its protein sequence places it in the multidrug resistance protein/cystic fibrosis transmembrane conductance regulator subfamily of ABC transporters. A key regulatory step in the biogenesis of this family of ABC transporter proteins is at the level of transport from the endoplasmic reticulum (ER) on through the secretory pathway. To explore the protein sequence requirements for Yor1p to move from the ER to its site of function at the plasma membrane, a series of truncation and alanine replacement mutations were constructed in Yor1p. This analysis detected two sequence motifs similar to the DXE element that has recently been found in other proteins that exit the ER. Loss of the N-terminal DXE element eliminated function of the protein, whereas loss of the C-terminal element only slightly reduced function of the resulting mutant Yor1p. Strikingly, although both of the single mutant proteins were stable, production of the double mutant caused dramatic destabilization of Yor1p. These data suggest that this large polytopic membrane protein requires multiple signals for normal forward trafficking, and elimination of this information may cause the mutant protein to be transferred to a degradative fate.

Amino Acid Sequence

Green Fluorescent Proteins

Endoplasmic Reticulum - metabolism

DNA Primers

Recombinant Fusion Proteins

Hydrolysis

Saccharomyces cerevisiae - metabolism

Base Sequence

Saccharomyces cerevisiae Proteins

ATP-Binding Cassette Transporters - metabolism

Signal Transduction - physiology

Microscopy, Fluorescence

Luminescent Proteins - metabolism

Details

Title: Subtitle: Identification of interdependent signals required for anterograde traffic of the ATP-binding cassette transporter protein Yor1p
Creators: Eric A Epping - Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242, USA
W Scott Moye-Rowley
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.277(38), pp.34860-34869
DOI: 10.1074/jbc.M202987200
PMID: 12107161
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: GM49825 / NIGMS NIH HHS T32 GM007337 / NIGMS NIH HHS
Language: English
Date published: 09/20/2002
Academic Unit: Molecular Physiology and Biophysics; Psychiatry; Internal Medicine
Record Identifier: 9984004091602771

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