Identification of lipidomic markers of chronic 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver

Izabela Kania-Korwel; Xianai Wu; Kai Wang; Hans-Joachim Lehmler

doi:10.1016/j.tox.2017.09.005

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Identification of lipidomic markers of chronic 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver

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Identification of lipidomic markers of chronic 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver

Izabela Kania-Korwel, Xianai Wu, Kai Wang and Hans-Joachim Lehmler

Toxicology (Amsterdam), Vol.390, pp.124-134

09/01/2017

DOI: 10.1016/j.tox.2017.09.005

PMCID: PMC5633524

PMID: 28890136

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Abstract

Exposure to PCB 126, an environmentally relevant aryl hydrocarbon receptor agonist, is an environmental factor causing hepatic steatosis in rodent models; however, the lipidome of PCB 126-exposed rats has not been investigated in-depth. The objective of the present study was therefore to characterize dose-dependent changes in the lipid profile in the liver of male Sprague-Dawley rats exposed to PCB 126. Rats were exposed for three month to intraperitoneal injections of 0.01, 0.05 and 0.2μmol/kg bw PCB 126 in corn oil. Control animals were exposed in parallel and received corn oil alone. Lipids were extracted from whole liver homogenate and levels of polar lipids and fatty acids incorporated into triglycerides (FATAGs) were determined with tandem mass spectrometry using electrospray ionization. PCB 126 exposure increased the hepatic content of polar lipids and FATAGs. Protein adjusted levels of several polar lipid classes, in particular phosphatidylserine levels, decreased, whereas FATAGs levels typically increased with increasing PCB 126 dose. Sensitive, dose-dependent endpoints of PCB 126 exposure included an increase in levels of adrenic acid incorporated into triglycerides and changes in levels of certain ether-linked phospholipid and 1-alkyl/1-alkenyldiacylglycerol species, as determined using partial least square discriminant analysis (PLS-DA) and ANOVA. These changes in the composition of polar lipids and fatty acid in the liver of PCB 126 exposed rats identified several novel markers of PCB 126-mediated fatty liver disease that need to be validated in further studies.

Lipidomics

Ether lipids

Polyunsaturated fatty acids

Phosphatidylserine

Diacyl glyceryl ethers

Adrenic acid

Synthesis Core

KC Donnelly colloboration

Details

Title: Subtitle: Identification of lipidomic markers of chronic 3,3′,4,4′,5-pentachlorobiphenyl (PCB 126) exposure in the male rat liver
Creators: Izabela Kania-Korwel - Department of Occupational Environmental Health, College of Public Health, The University of Iowa, Iowa City, IA, USA
Xianai Wu - Department of Occupational Environmental Health, College of Public Health, The University of Iowa, Iowa City, IA, USA
Kai Wang - Department of Biostatistics, College of Public Health, The University of Iowa, Iowa City, IA, USA
Hans-Joachim Lehmler - Department of Occupational Environmental Health, College of Public Health, The University of Iowa, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Toxicology (Amsterdam), Vol.390, pp.124-134
DOI: 10.1016/j.tox.2017.09.005
PMID: 28890136
PMCID: PMC5633524
NLM abbreviation: Toxicology
ISSN: 0300-483X
eISSN: 1879-3185
Publisher: Elsevier B.V
Language: English
Date published: 09/01/2017
Academic Unit: Occupational and Environmental Health; Iowa Neuroscience Institute; Biostatistics; Iowa Superfund Research Program
Record Identifier: 9983997302602771

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