Identification of novel ARF binding proteins by two-hybrid screening

Van Tompkins; Jussara Hagen; Valerie P Zediak; Dawn E Quelle

doi:10.4161/cc.5.6.2560

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Identification of novel ARF binding proteins by two-hybrid screening

Journal article

Open access

Peer reviewed

Identification of novel ARF binding proteins by two-hybrid screening

Van Tompkins, Jussara Hagen, Valerie P Zediak and Dawn E Quelle

Cell cycle (Georgetown, Tex.), Vol.5(6), pp.641-647

03/2006

DOI: 10.4161/cc.5.6.2560

PMID: 16582619

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Abstract

The ARF tumor suppressor protects us against cancer through protein-protein interactions in partially defined p53-dependent and p53-independent pathways. We performed a two-hybrid screen using ARF as bait and present the identification of several new ARF partners that may regulate its growth inhibitory signaling. The potential physiological roles of these novel ARF binding proteins in regulating ARF signaling are discussed.

NIH 3T3 Cells

Protein Binding - genetics

Humans

Cercopithecus aethiops

Signal Transduction - genetics

Cell Transformation, Neoplastic - metabolism

Cyclin-Dependent Kinase Inhibitor p16

Growth Inhibitors - metabolism

Carrier Proteins - genetics

Two-Hybrid System Techniques

Animals

Carrier Proteins - metabolism

Cell Transformation, Neoplastic - genetics

Carrier Proteins - isolation & purification

Mice

Tumor Suppressor Protein p14ARF - genetics

Growth Inhibitors - genetics

Tumor Suppressor Protein p14ARF - metabolism

COS Cells

Details

Title: Subtitle: Identification of novel ARF binding proteins by two-hybrid screening
Creators: Van Tompkins - Department of Pharmacology, The University of Iowa, College of Medicine, Iowa City 52242, USA
Jussara Hagen
Valerie P Zediak
Dawn E Quelle
Resource Type: Journal article
Publication Details: Cell cycle (Georgetown, Tex.), Vol.5(6), pp.641-647
DOI: 10.4161/cc.5.6.2560
PMID: 16582619
NLM abbreviation: Cell Cycle
ISSN: 1538-4101
eISSN: 1551-4005
Publisher: United States
Grant note: R01 CA090367 / NCI NIH HHS
Language: English
Date published: 03/2006
Academic Unit: Molecular Physiology and Biophysics; Pathology; Neuroscience and Pharmacology
Record Identifier: 9984040450902771

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