Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae

Xin-Jian He; Jan S Fassler

doi:10.1111/j.1365-2958.2005.04917.x

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Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae

Journal article

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Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae

Xin-Jian He and Jan S Fassler

Molecular microbiology, Vol.58(5), pp.1454-1467

12/2005

DOI: 10.1111/j.1365-2958.2005.04917.x

PMCID: PMC2916641

PMID: 16313629

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Abstract

The Saccharomyces cerevisiae Yap1p and Skn7p transcription factors collaborate in the activation of oxidative stress response (OSR) genes. Although Yap1p and Skn7p oxidative stress response elements (YRE, OSRE) have been characterized and identified in some OSR genes, many OSR genes lack such elements. In this study, the complex, oxidative responsive, CCP1 promoter was used as a model to investigate the cis-acting elements responsible for activation by oxidative stress. In addition to consensus YRE and OSRE sequences, novel Yap1p and Skn7p binding sites were identified in the CCP1 promoter. These new sites were found to mediate Yap1p- and Skn7p-dependent activation of OSR genes including TSA1 and CTT1 previously thought to lack Yap1p and Skn7p binding sites. The novel YREs and OSREs were found to be enriched in the promoter regions of a set of 179 OSR genes. The widespread existence of novel Yap1p and Skn7p binding sites strongly suggest that direct binding of Yap1p and Skn7p is responsible for activation of many more OSR genes than previously believed.

Base Sequence

Binding Sites - genetics

DNA-Binding Proteins - chemistry

DNA-Binding Proteins - metabolism

Enhancer Elements, Genetic - genetics

Gene Expression Regulation, Fungal

Heat-Shock Response

Molecular Sequence Data

Oxidative Stress

Promoter Regions, Genetic

Saccharomyces cerevisiae - genetics

Saccharomyces cerevisiae - physiology

Saccharomyces cerevisiae Proteins - chemistry

Saccharomyces cerevisiae Proteins - genetics

Saccharomyces cerevisiae Proteins - metabolism

Transcription Factors - chemistry

Transcription Factors - metabolism

Details

Title: Subtitle: Identification of novel Yap1p and Skn7p binding sites involved in the oxidative stress response of Saccharomyces cerevisiae
Creators: Xin-Jian He - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA
Jan S Fassler
Resource Type: Journal article
Publication Details: Molecular microbiology, Vol.58(5), pp.1454-1467
DOI: 10.1111/j.1365-2958.2005.04917.x
PMID: 16313629
PMCID: PMC2916641
ISSN: 0950-382X
eISSN: 1365-2958
Grant note: R01 GM056719 / NIGMS NIH HHS R01 GM056719-08 / NIGMS NIH HHS GM56719 / NIGMS NIH HHS
Language: English
Date published: 12/2005
Academic Unit: Biology
Record Identifier: 9984217414502771

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