Journal article
Identification of protein oligomerization states by analysis of interface conservation
Proceedings of the National Academy of Sciences - PNAS, Vol.98(6), pp.2990-2994
03/06/2001
DOI: 10.1073/pnas.061411798
PMCID: PMC30594
PMID: 11248019
Abstract
The discrimination of true oligomeric protein–protein
contacts from nonspecific crystal contacts remains problematic.
Criteria that have been used previously base the assignment of
oligomeric state on consideration of the area of the interface and/or
the results of scoring functions based on statistical potentials. Both
techniques have a high success rate but fail in more than 10% of
cases. More importantly, the oligomeric states of several proteins are
incorrectly assigned by both methods. Here we test the hypothesis that
true oligomeric contacts should be identifiable on the basis of an
increased degree of conservation of the residues involved in the
interface. By quantifying the degree of conservation of the interface
and comparing it with that of the remainder of the protein surface, we
develop a new criterion that provides a highly effective complement to
existing methods.
Details
- Title: Subtitle
- Identification of protein oligomerization states by analysis of interface conservation
- Creators
- Adrian H ElcockJ. Andrew Mccammon
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.98(6), pp.2990-2994
- DOI
- 10.1073/pnas.061411798
- PMID
- 11248019
- PMCID
- PMC30594
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 03/06/2001
- Academic Unit
- Physics and Astronomy; Biochemistry and Molecular Biology
- Record Identifier
- 9984025287402771
Metrics
11 Record Views