Journal article
Identification of rfk-1 , a Meiotic Driver Undergoing RNA Editing in Neurospora
Genetics (Austin), Vol.212(1), pp.93-110
05/2019
DOI: 10.1534/genetics.119.302122
PMCID: PMC6499513
PMID: 30918007
Abstract
is a meiotic drive element that was discovered in wild populations of
fungi over 40 years ago. While early studies quickly determined that
transmits itself through sexual reproduction in a biased manner via spore killing, the genetic factors responsible for this phenomenon have remained mostly unknown. Here, we identify and characterize
, a gene required for
-based spore killing. The
gene contains four exons, three introns, and two stop codons, the first of which undergoes RNA editing to a tryptophan codon during sexual development. Translation of an unedited
transcript in vegetative tissue is expected to produce a 102-amino acid protein, whereas translation of an edited
transcript in sexual tissue is expected to produce a protein with 130 amino acids. These findings indicate that unedited and edited
transcripts exist and that these transcripts could have different roles with respect to the mechanism of meiotic drive by spore killing. Regardless of RNA editing, spore killing only succeeds if
transcripts avoid silencing caused by a genome defense process called meiotic silencing by unpaired DNA (MSUD). We show that
's MSUD avoidance mechanism is linked to the genomic landscape surrounding the
gene, which is located near the
border on the right arm of chromosome III. In addition to demonstrating that the location of
is critical to spore-killing success, our results add to accumulating evidence that MSUD helps protect
genomes from complex meiotic drive elements.
Details
- Title: Subtitle
- Identification of rfk-1 , a Meiotic Driver Undergoing RNA Editing in Neurospora
- Creators
- Nicholas A Rhoades - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Austin M Harvey - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Dilini A Samarajeewa - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Jesper Svedberg - Department of Organismal Biology, Uppsala University, Norbyvägen 18D, 752 36 Uppsala, SwedenAykhan Yusifov - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Anna Abusharekh - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Pennapa Manitchotpisit - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Daren W Brown - Mycotoxin Prevention and Applied Microbiology, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, Peoria, Illinois 61604Kevin J Sharp - School of Biological Sciences, Illinois State University, Normal, Illinois 61790David G Rehard - Department of Biology, University of Iowa, Iowa 52242Joshua Peters - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Xavier Ostolaza-Maldonado - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Jackson Stephenson - School of Biological Sciences, Illinois State University, Normal, Illinois 61790Patrick K T Shiu - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211Hanna Johannesson - Department of Organismal Biology, Uppsala University, Norbyvägen 18D, 752 36 Uppsala, SwedenThomas M Hammond - School of Biological Sciences, Illinois State University, Normal, Illinois 61790 tmhammo@ilstu.edu
- Resource Type
- Journal article
- Publication Details
- Genetics (Austin), Vol.212(1), pp.93-110
- DOI
- 10.1534/genetics.119.302122
- PMID
- 30918007
- PMCID
- PMC6499513
- ISSN
- 0016-6731
- eISSN
- 1943-2631
- Grant note
- P01 GM068087 / NIGMS NIH HHS
- Language
- English
- Date published
- 05/2019
- Academic Unit
- Biology
- Record Identifier
- 9984217525702771
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