Journal article
Identification of tandem genes involved in lipooligosaccharide expression by Haemophilus ducreyi
Infection and immunity, Vol.65(2), pp.651-660
02/1997
DOI: 10.1128/iai.65.2.651-660.1997
PMCID: PMC176110
PMID: 9009327
Abstract
A transposon insertion mutant of Haemophilus ducreyi 35000 possessing a truncated lipooligosaccharide (LOS) failed to bind the LOS-specific monoclonal antibody 3E6 (M. K. Stevens, L. D. Cope, J. D. Radolf, and E. J. Hansen, Infect. Immun. 63:2976-2982, 1995). This transposon was found to have inserted into the first of two tandem genes and also caused a deletion of chromosomal DNA upstream of this gene. These two genes, designated lbgA and lbgB, encoded predicted proteins with molecular masses of 25,788 and 40,236 Da which showed homology with proteins which function in lipopolysaccharide biosynthetic in other gram-negative bacteria. The tandem arrangement of the lbgA and lbgB genes was found to be conserved among H. ducreyi strains. Isogenic LOS mutants, constructed by the insertion of a cat cartridge into either the lbgA or the lbgB gene, expressed an LOS phenotype indistinguishable from that of the original transposon-derived LOS mutant. The wild-type LOS phenotype could be restored by complementation with the appropriate wild-type allele. These two LOS mutants proved to be as virulent as the wild-type parent strain in an animal model. A double mutant with a deletion of the lbgA and lbgB genes yielded equivocal results when its virulence was tested in an animal model.
Details
- Title: Subtitle
- Identification of tandem genes involved in lipooligosaccharide expression by Haemophilus ducreyi
- Creators
- Marla K Stevens - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USAJulia Klesney-Tait - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USASheryl Lumbley - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USAK A Walters - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USAA Mark Joffe - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USAJustin D Radolf - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USAEric J Hansen - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235-9048, USA
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.65(2), pp.651-660
- DOI
- 10.1128/iai.65.2.651-660.1997
- PMID
- 9009327
- PMCID
- PMC176110
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 02/1997
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984094710602771
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