Identification of the authentic varicella-zoster virus gB (gene 31) initiating methionine overlapping the 3′ end of gene 30

Lucie Maresova; TracyJo Pasieka; Timothy Wagenaar; Wallen Jackson; Charles Grose

doi:10.1002/jmv.10324

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Identification of the authentic varicella-zoster virus gB (gene 31) initiating methionine overlapping the 3′ end of gene 30

Journal article

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Identification of the authentic varicella-zoster virus gB (gene 31) initiating methionine overlapping the 3′ end of gene 30

Lucie Maresova, TracyJo Pasieka, Timothy Wagenaar, Wallen Jackson and Charles Grose

Journal of medical virology, Vol.70(S1), pp.S64-S70

2003

DOI: 10.1002/jmv.10324

PMID: 12627491

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Abstract

The varicella-zoster virus (VZV) gB sequence was re-examined in light of recent knowledge about unusually long gB signal peptides in other herpesviral gB homologs. Through mutational analysis, the discovery was made that the authentic initiating methionine for VZV gB is a codon beginning at genome nucleotide 56,819. The total length for the VZV gB primary translation product was 931 amino acids (aa) with a 71-aa signal sequence. Considering the likely signal sequence cleavage site to be located between Ser 71 and Val 72, the length of the mature VZV gB polypeptide would then be 860 amino acids prior to further internal endoproteolytic cleavage between amino acids Arg 494 and Ser 495. In this report, we also produced a full-length gB and demonstrated its association with VZV gE, suggesting a possible gE-gB interaction during gB trafficking before its cleavage in the Golgi.

VZV gB sequence

VZV gE

signal peptides

Details

Title: Subtitle: Identification of the authentic varicella-zoster virus gB (gene 31) initiating methionine overlapping the 3′ end of gene 30
Creators: Lucie Maresova - Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, Iowa
TracyJo Pasieka - Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, Iowa
Timothy Wagenaar - Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, Iowa
Wallen Jackson - Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, Iowa
Charles Grose - Departments of Microbiology and Pediatrics, University of Iowa, Iowa City, Iowa
Resource Type: Journal article
Publication Details: Journal of medical virology, Vol.70(S1), pp.S64-S70
Publisher: Wiley Subscription Services, Inc., A Wiley Company
DOI: 10.1002/jmv.10324
PMID: 12627491
ISSN: 0146-6615
eISSN: 1096-9071
Number of pages: 7
Language: English
Date published: 2003
Academic Unit: Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093336702771

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