Identification of the spinal cord as a major site of persistence during during chronic infection with a murine coronavirus

Stanley Perlman; Gary Jacobsen; Ann Louise Olson; Adel  Afifi

doi:10.1016/0042-6822(90)90426-r

Back

Identification of the spinal cord as a major site of persistence during during chronic infection with a murine coronavirus

Journal article

Open access

Peer reviewed

Identification of the spinal cord as a major site of persistence during during chronic infection with a murine coronavirus

Stanley Perlman, Gary Jacobsen, Ann Louise Olson and Adel Afifi

Virology, Vol.175(2), pp.418-426

04/1990

DOI: 10.1016/0042-6822(90)90426-r

PMCID: PMC7131507

PMID: 2158180

Files and links (1)

url

https://doi.org/10.1016/0042-6822(90)90426-RView

Published (Version of record) Open Access

Abstract

After intranasal inoculation, mouse hepatitis virus (MHV) gains entry into the central nervous system (CNS) via the olfactory and trigeminal nerves. Under the appropriate conditions, some mice develop clinically apparent demyelinating encephalomyelitis several weeks later, with virus always present in the spinal cord. To determine the pathway by which virus reaches the cord, brains and spinal cords of infected, asymptomatic mice were analyzed by in situ hybridization. Viral RNA was always detected in the anterior part of the upper spinal cord. A similar analysis of mice with the recent onset of hindlimb weakness showed that viral RNA was detected in the same location. The results suggest that MHV is transported to the spinal cord via well-defined neuroanatomic pathways and that viral amplification with resultant clinical disease occurs from this site of persistence in the anterior spinal cord. This process of viral amplification may involve the generation of viral variants as has been described for MHV-infected rats. No major changes in viral RNA or protein could be detected when MHV isolated from mice with hindlimb paralysis was analyzed. The data suggest that the generation of viral variants is not important in the pathogenesis of the late onset of neurological disease induced by MHV in mice.

Nucleic Acid Hybridization

Hindlimb

Brain - microbiology

Mice, Inbred C57BL

RNA, Viral - analysis

L Cells (Cell Line)

Paralysis - microbiology

Coronaviridae Infections - microbiology

Murine hepatitis virus - physiology

Murine hepatitis virus - isolation & purification

RNA Probes

Animals

Virus Replication

Spinal Cord - microbiology

Mice

RNA, Viral - isolation & purification

Chronic Disease

Murine hepatitis virus - genetics

Details

Title: Subtitle: Identification of the spinal cord as a major site of persistence during during chronic infection with a murine coronavirus
Creators: Stanley Perlman - Department of Pediatrics, University of Iowa, Iowa City 52242
Gary Jacobsen
Ann Louise Olson
Adel Afifi - University of Iowa, Stead Family Department of Pediatrics
Resource Type: Journal article
Publication Details: Virology, Vol.175(2), pp.418-426
DOI: 10.1016/0042-6822(90)90426-r
PMID: 2158180
PMCID: PMC7131507
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Published by Elsevier Inc; United States
Grant note: NS24401 / NINDS NIH HHS
Language: English
Date published: 04/1990
Academic Unit: Preventive and Community Dentistry; Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777346902771

Metrics

27 Record Views

54 Times Cited - Web of Science