Journal article
Identification of the znuA-Encoded Periplasmic Zinc Transport Protein of Haemophilus ducreyi
Infection and immunity, Vol.67(10), pp.5060-5068
10/1999
DOI: 10.1128/iai.67.10.5060-5068.1999
PMCID: PMC96853
PMID: 10496878
Abstract
The
znuA
gene of
Haemophilus ducreyi
encodes a 32-kDa (mature) protein that has homology to both the ZnuA protein of
Escherichia coli
and the Pzp1 protein of
H. influenzae
; both of these latter proteins are members of a growing family of prokaryotic zinc transporters. Inactivation of the
H. ducreyi
35000
znuA
gene by insertional mutagenesis resulted in a mutant that grew more slowly than the wild-type parent strain in vitro unless ZnCl
2
was provided at a final concentration of 100 μM. Other cations tested did not restore growth of this
H. ducreyi
mutant to wild-type levels. The
H. ducreyi
ZnuA protein was localized to the periplasm, where it is believed to function as the binding component of a zinc transport system. Complementation of the
znuA
mutation with the wild-type
H. ducreyi znuA
gene provided in
trans
restored the ability of this
H. ducreyi
mutant to grow normally in the absence of exogenously added ZnCl
2
. The wild-type
H. ducreyi znuA
gene was also able to complement a
H. influenzae pzp1
mutation. The
H. ducreyi znuA
isogenic mutant exhibited significantly decreased virulence (
P
= 0.0001) when tested in the temperature-dependent rabbit model for experimental chancroid. This decreased virulence was not observed when the
znuA
mutant was complemented with the wild-type
H. ducreyi znuA
gene provided in
trans
.
Details
- Title: Subtitle
- Identification of the znuA-Encoded Periplasmic Zinc Transport Protein of Haemophilus ducreyi
- Creators
- David A Lewis - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9048Julia Klesney-Tait - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9048Sheryl R Lumbley - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9048Christine K Ward - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9048Jo L Latimer - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9048Catherine A Ison - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9048Eric J Hansen - Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, Texas 75235-9048
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.67(10), pp.5060-5068
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/iai.67.10.5060-5068.1999
- PMID
- 10496878
- PMCID
- PMC96853
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 10/1999
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984094711902771
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