Journal article
Identifying novel ovarian tumor biomarkers through mining of the transcriptome of circulating immune cells: A proof-of-concept study
American journal of reproductive immunology (1989), Vol.86(4), pp.e13469-n/a
10/01/2021
DOI: 10.1111/aji.13469
PMID: 34022086
Abstract
Objective Treatment of high-grade serous ovarian cancer (HGSOC) will benefit from early detection of cancer. Here, we provide proof-of-concept data supporting the hypothesis that circulating immune cells, because of their early recognition of tumors and the tumor microenvironment, can be considered for biomarker discovery. Methods Longitudinal blood samples from C57BL/6 mice bearing syngeneic ovarian tumors and peripheral blood mononuclear cells (PBMC) from healthy postmenopausal women and newly diagnosed for HGSOC patients were subjected to RNASeq. The results from human immune cells were validated using Affymetrix microarrays. Differentially expressed transcripts in immune cells from tumor-bearing mice and HGSOC patients were compared to matching controls. Results A total of 1282 transcripts (798 and 484, up- and downregulated, respectively) were differentially expressed in the tumor-bearing mice as compared with controls. Top 100 genes showing longitudinal changes in gene expression 2, 4, 7, and 18 days after tumor implantation were identified. Analysis of the PBMC from healthy post-menopausal women and HGSOC patients identified 4382 differentially expressed genes and 519 of these were validated through Affymetrix microarray analysis. A total of 384 genes, including IL-1R2, CH3L1, Infitm1, FP42, CXC42, Hdc, Spib, and Sema6b, were differentially expressed in the human and mouse datasets. Conclusion The PBMC transcriptome shows longitudinal changes in response to the progressing tumor. Several potential biomarker transcripts were identified in HGSOC patients and mouse models. Monitoring their expression in individual PBMC subsets can serve as additional discriminator for the diagnosis of HGSOC.
Details
- Title: Subtitle
- Identifying novel ovarian tumor biomarkers through mining of the transcriptome of circulating immune cells: A proof-of-concept study
- Creators
- Shitanshu Uppal - University of Wisconsin–MadisonErin Medlin - University of Wisconsin–MadisonMildred Felder - University of Wisconsin–MadisonArvinder Kapur - University of Wisconsin–MadisonRobert C. Sterner - Univ Wisconsin, Dept Obstet & Gynecol, Madison, WI 53706 USAJessica Vazquez - University of Wisconsin–MadisonPayton N. Lindner - University of Wisconsin–MadisonAleksandar K. Stanic - University of Wisconsin–MadisonLisa Barroilhet - University of Wisconsin–MadisonJesus Gonzalez-Bosquet - University of IowaManish S. Patankar - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- American journal of reproductive immunology (1989), Vol.86(4), pp.e13469-n/a
- Publisher
- Wiley
- DOI
- 10.1111/aji.13469
- PMID
- 34022086
- ISSN
- 1046-7408
- eISSN
- 1600-0897
- Number of pages
- 8
- Grant note
- W81XWH-11-1-0181; 598088 / Department of Defense Ovarian Cancer Research Program; United States Department of Defense University of Wisconsin Comprehensive Cancer Center Department of Obstetrics and Gynecology CA14520 / Wisconsin Ovarian Cancer Alliance
- Language
- English
- Date published
- 10/01/2021
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9984315744302771
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