Image analysis for quantitation of solid tumor drug sensitivity

J F Gibbs; H K Slocum; S Cao; Y M Rustum

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Journal article

Image analysis for quantitation of solid tumor drug sensitivity

J F Gibbs, H K Slocum, S Cao and Y M Rustum

International journal of surgical investigation, Vol.1(2), pp.133-138

1999

PMID: 11341633

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Abstract

A method of assessing chemosensitivity of tissue has been described by Rotman et al. The aim of this study was to use image analysis to provide a more rapid and quantitative means of assessing drug effect on tissue proliferative capacity. Fluoropyrimidine sensitive Ward rat colon adenocarcinoma tumor was implanted onto collagen impregnated cellulose fibers suspended on metal grids at an air-fluid interface and kept in a 95% air/5% CO2 incubator at 37 degrees C. The fluorescent microscopic image captured by a silicon intensified target (low light detecting) camera and linked to an image processing unit was measured for fluorescent brightness and tumor image area. Blinded 5-Fluorouracil (5-FU) drug treatment was begun 8 days after tumor explantation on the collagen-cellulose matrix. Tumor image area and fluorescent brightness were measured at 24 h pretreatment, 48 h posttreatment, and at 48 h post drug removal. Nontreated tumor cultures demonstrated an increase in area and fluorescent brightness with time following tumor implantation on the collagen gel. Dose responsiveness was seen with increasing concentrations of 5-FU. At the highest clinically achievable concentration of 5-FU (500 microM), there was a 39% decrease in area compared with the nontreated group, 113%. Linear dose responsiveness was not demonstrated between 50 and 150microM 5-FU. Fluoropyrimidine activity was demonstrated with the implemented image analysis system. The in vitro tumor sensitivity to FU using collagen gel was consistent with responsiveness of tumors in vivo borne by rats.

Adenocarcinoma - metabolism

Animals

Antimetabolites, Antineoplastic - pharmacology

Colonic Neoplasms - metabolism

Dose-Response Relationship, Drug

Drug Resistance, Neoplasm

Fluorouracil - pharmacology

Image Processing, Computer-Assisted

Microscopy, Fluorescence

Osmolar Concentration

Pyrimidines - pharmacokinetics

Rats

Single-Blind Method

Details

Title: Subtitle: Image analysis for quantitation of solid tumor drug sensitivity
Creators: J F Gibbs - Department of Surgical Oncology, Roswell Park Cancer Institute, State University of New York at Buffalo, 14263, USA. jgibbs@sc3101.med.buffalo.edu
H K Slocum
S Cao
Y M Rustum
Resource Type: Journal article
Publication Details: International journal of surgical investigation, Vol.1(2), pp.133-138
PMID: 11341633
ISSN: 1028-5229
Grant note: CA 16056 / NCI NIH HHS CA 09581 / NCI NIH HHS
Language: English
Date published: 1999
Academic Unit: Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
Record Identifier: 9984359668902771

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