Image analysis using the fluorochromasia assay to quantify tumor drug sensitivity

John F Gibbs; Youcef M Rustum; Harry K Slocum

doi:10.1385/1-59259-869-2:185

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Image analysis using the fluorochromasia assay to quantify tumor drug sensitivity

Journal article

Peer reviewed

Image analysis using the fluorochromasia assay to quantify tumor drug sensitivity

John F Gibbs, Youcef M Rustum and Harry K Slocum

Methods in molecular medicine, Vol.110, pp.185-195

2005

DOI: 10.1385/1-59259-869-2:185

PMID: 15901936

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Abstract

A method of assessing chemosensitivity of tissue utilizing tissue fluorescence and image analysis was implemented to provide a rapid and quantitative means of assessing the effect of drugs on tissue metabolic activity and proliferative capacity. The fluorescent microscopic image captured by a silicon-intensified target (low-light-detecting) camera and linked to an image- processing unit was measured for fluorescent brightness and tumor image area. An established rodent model served to characterize the system's ability to measure serially the tumor's metabolic activity and growth. Further studies on fresh human tumors were conducted with a novel topoisomerase II inhibitor, NC-190. Tumor image area and fluorescent brightness were measured 24 h pretreatment, 48 h posttreatment, and 48 h post-drug removal. Fifty-five percent (28/51) of fresh human tumors showed sensitivity to 48-h exposure to 10, 30, or 100 microM NC-190. The potential benefit of this technique is the ability to predict the response of tumors to chemotherapeutic agents as a laboratory tool for preclinical drug evaluation and clinically prior to the commencement of therapy.

1,2-Dimethylhydrazine

Adenocarcinoma - chemically induced

Adenocarcinoma - metabolism

Adenocarcinoma - pathology

Animals

Antineoplastic Agents - pharmacology

Biopsy

Cell Survival - drug effects

Colonic Neoplasms - chemically induced

Colonic Neoplasms - metabolism

Colonic Neoplasms - pathology

Drug Screening Assays, Antitumor - methods

Fluoresceins - metabolism

Fluorescent Dyes - metabolism

Humans

Image Processing, Computer-Assisted

Jejunal Neoplasms - chemically induced

Jejunal Neoplasms - metabolism

Jejunal Neoplasms - pathology

Microscopy, Fluorescence

Neoplasms - metabolism

Neoplasms - pathology

Phenazines - pharmacology

Rats

Topoisomerase II Inhibitors

Details

Title: Subtitle: Image analysis using the fluorochromasia assay to quantify tumor drug sensitivity
Creators: John F Gibbs - Roswell Park Cancer Institute
Youcef M Rustum - Roswell Park Cancer Institute
Harry K Slocum - Roswell Park Cancer Institute
Resource Type: Journal article
Publication Details: Methods in molecular medicine, Vol.110, pp.185-195
DOI: 10.1385/1-59259-869-2:185
PMID: 15901936
eISBN: 9781592598694; 1592598692
ISSN: 1543-1894
eISSN: 1940-6037
Language: English
Date published: 2005
Academic Unit: Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
Record Identifier: 9984360042102771

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