Image-based multiscale modeling predicts tissue-level and network-level fiber reorganization in stretched cell-compacted collagen gels

Edward A Sander; Triantafyllos Stylianopoulos; Robert T Tranquillo; Victor H Barocas

doi:10.1073/pnas.0903716106

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Image-based multiscale modeling predicts tissue-level and network-level fiber reorganization in stretched cell-compacted collagen gels

Journal article

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Image-based multiscale modeling predicts tissue-level and network-level fiber reorganization in stretched cell-compacted collagen gels

Edward A Sander, Triantafyllos Stylianopoulos, Robert T Tranquillo and Victor H Barocas

Proceedings of the National Academy of Sciences - PNAS, Vol.106(42), pp.17675-17680

10/20/2009

DOI: 10.1073/pnas.0903716106

PMCID: PMC2764876

PMID: 19805118

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Abstract

The mechanical environment plays an important role in cell signaling and tissue homeostasis. Unraveling connections between externally applied loads and the cellular response is often confounded by extracellular matrix (ECM) heterogeneity. Image-based multiscale models provide a foundation for examining the fine details of tissue behavior, but they require validation at multiple scales. In this study, we developed a multiscale model that captured the anisotropy and heterogeneity of a cell-compacted collagen gel subjected to an off-axis hold mechanical test and subsequently to biaxial extension. In both the model and experiments, the ECM reorganized in a nonaffine and heterogeneous manner that depended on multiscale interactions between the fiber networks. Simulations predicted that tensile and compressive fiber forces were produced to accommodate macroscopic displacements. Fiber forces in the simulation ranged from −11.3 to 437.7 nN, with a significant fraction of fibers under compression (12.1% during off-axis stretch). The heterogeneous network restructuring predicted by the model serves as an example of how multiscale modeling techniques provide a theoretical framework for understanding relationships between ECM structure and tissue-level mechanical properties and how microscopic fiber rearrangements could lead to mechanotransductive cell signaling.

mechanobiology

Biological Sciences

Physical Sciences

cruciforms

biomechanics

tissue mechanics

Details

Title: Subtitle: Image-based multiscale modeling predicts tissue-level and network-level fiber reorganization in stretched cell-compacted collagen gels
Creators: Edward A Sander - Departments of
Triantafyllos Stylianopoulos - Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN 55455
Robert T Tranquillo - Departments of
Victor H Barocas - Departments of
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.106(42), pp.17675-17680
DOI: 10.1073/pnas.0903716106
PMID: 19805118
PMCID: PMC2764876
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 10/20/2009
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Chemical and Biochemical Engineering
Record Identifier: 9984064588402771

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