Journal article
Imaging Olfactory Learning-Induced Plasticity in Vivo in the Drosophila Brain
Cold Spring Harbor protocols, Vol.2024(6), pdb.prot108135
06/03/2024
DOI: 10.1101/pdb.prot108135
PMID: 37197829
Abstract
In vivo imaging of brain activity in
allows the dissection of numerous types of biologically important neuronal events. A common paradigm involves imaging neuronal Ca
transients, often in response to sensory stimuli. These Ca
transients correlate with neuronal spiking activity, which generates voltage-sensitive Ca
influx. In addition, there is a range of genetically encoded reporters of membrane voltage and of other signaling molecules, such as second-messenger signaling cascade enzymes and neurotransmitters, enabling optical access to a range of cellular processes. Moreover, sophisticated gene expression systems enable access to virtually any single neuron or neuronal group in the fly brain. The in vivo imaging approach enables the study of these processes and how they change during salient sensory-driven events such as olfactory associative learning, when an animal (fly) is presented an odor (a conditioned stimulus) paired with an unconditioned stimulus (an aversive or appetitive stimulus) and forms an associative memory of this pairing. Optical access to neuronal events in the brain allows one to image learning-induced plasticity following the formation of associative memory, dissecting the mechanisms of memory formation, maintenance, and recall.
Details
- Title: Subtitle
- Imaging Olfactory Learning-Induced Plasticity in Vivo in the Drosophila Brain
- Creators
- Tamara Boto - Trinity College DublinSeth M Tomchik - Iowa Neuroscience Institute, University of Iowa Carver College of Medicine, Iowa City, Iowa 52242, USA
- Resource Type
- Journal article
- Publication Details
- Cold Spring Harbor protocols, Vol.2024(6), pdb.prot108135
- DOI
- 10.1101/pdb.prot108135
- PMID
- 37197829
- NLM abbreviation
- Cold Spring Harb Protoc
- ISSN
- 1559-6095
- eISSN
- 1559-6095
- Language
- English
- Electronic publication date
- 05/17/2023
- Date published
- 06/03/2024
- Academic Unit
- Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984413075802771
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