Imaging calcium sparks in cardiac myocytes

Silvia Guatimosim; Cristina Guatimosim; Long-Sheng Song

doi:10.1007/978-1-60761-950-5_12

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Journal article

Imaging calcium sparks in cardiac myocytes

Silvia Guatimosim, Cristina Guatimosim and Long-Sheng Song

Methods in molecular biology (Clifton, N.J.), Vol.689, pp.205-214

2011

DOI: 10.1007/978-1-60761-950-5_12

PMCID: PMC3233356

PMID: 21153794

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https://www.ncbi.nlm.nih.gov/pmc/articles/3233356View

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Abstract

Calcium ions play fundamental roles in many cellular processes in virtually all type of cells. The use of Ca(2+) sensitive fluorescent indicators has proven to be an indispensable tool for studying the spatio-temporal dynamics of intracellular calcium ([Ca(2+)](i)). With the aid of laser scanning confocal microscopy and new generation of Ca(2+) indicators, highly localized, short-lived Ca(2+) signals, namely Ca(2+) sparks, were revealed as elementary Ca(2+) release events during excitation-contraction coupling in cardiomyocytes. Since the discovery of Ca(2+) sparks in 1993, the demonstration of dynamic Ca(2+) micro-domains in living cardiomyocytes has revolutionized our understanding of Ca(2+)-mediated signal transduction in normal and diseased hearts. In this chapter, we have described a commonly used method for recording local and global Ca(2+) signals in cardiomyocytes using the fluorescent indicator fluo-4 acetoxymethyl (AM) and laser scanning confocal microscopy.

Aniline Compounds

Calcium Signaling

Fluorescent Dyes

Microscopy, Confocal - methods

Muscle Contraction - physiology

Myocytes, Cardiac - cytology

Signal Transduction - physiology

Xanthenes

Details

Title: Subtitle: Imaging calcium sparks in cardiac myocytes
Creators: Silvia Guatimosim - Universidade Federal de Minas Gerais
Cristina Guatimosim - Universidade Federal de Minas Gerais
Long-Sheng Song - Roy J. and Lucille A. Carver College of Medicine
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.689, pp.205-214
DOI: 10.1007/978-1-60761-950-5_12
PMID: 21153794
PMCID: PMC3233356
NLM abbreviation: Methods Mol Biol
eISBN: 9781607619505; 1607619504
ISSN: 1064-3745
eISSN: 1940-6029
Grant note: R01 HL090905-02S1 / NHLBI NIH HHS R01 HL090905-02 / NHLBI NIH HHS R01 HL090905-04 / NHLBI NIH HHS R01 HL090905 / NHLBI NIH HHS R01 HL090905-03 / NHLBI NIH HHS R01 HL090905-01 / NHLBI NIH HHS
Language: English
Date published: 2011
Academic Unit: Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984288725602771

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