Imaging free zinc in synaptic terminals in live hippocampal slices

T Budde; A Minta; J A White; A.R Kay

doi:10.1016/S0306-4522(96)00695-1

Back

Imaging free zinc in synaptic terminals in live hippocampal slices

Journal article

Open access

Peer reviewed

Imaging free zinc in synaptic terminals in live hippocampal slices

T Budde, A Minta, J A White and A.R Kay

Neuroscience, Vol.79(2), pp.347-358

1997

DOI: 10.1016/S0306-4522(96)00695-1

PMID: 9200720

Files and links (1)

url

https://doi.org/10.1016/S0306-4522(96)00695-1View

Published (Version of record) Open Access

Abstract

Some glutamatergic synapses in the mammalian central nervous system exhibit high levels of free ionic zinc in their synaptic vesicles. The precise role of this vesicular zinc remains obscure, despite suggestive evidence for zinc as a neuromodulator. As a step towards elucidating the role of free zinc in the brain we have developed a method for imaging zinc release in live brain slices. A newly synthesized zinc-sensitive fluorescent probe, N-(6-methoxy-8-quinolyl)-p-carboxybenzoylsulphonamide (TFLZn), was used to monitor intracellular zinc in live rat hippocampal slices. The dye loaded into the zinc-rich synaptic vesicles of the mossy fibre terminals in the hippocampal formation. Direct electrical stimulation of the mossy fibre pathway diminished the fluorescence in the mossy fibre terminals, consistent with a stimulus-dependent zinc release. The synaptic release of zinc was followed by the rapid replenishment of the zinc levels in vesicles from an as yet unidentified intracellular zinc source. Furthermore, we present evidence that zinc may play a role in a form of long-term potentiation exhibited by the mossy fibre pathway.

Zinc

mossy fibres

synapses

hippocampus

synaptic vesicles

imaging

Details

Title: Subtitle: Imaging free zinc in synaptic terminals in live hippocampal slices
Creators: T Budde - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, U.S.A
A Minta - Texas Fluorescence Laboratories, Austin, TX 78747, U.S.A
J A White - Boston University
A.R Kay - Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, U.S.A
Resource Type: Journal article
Publication Details: Neuroscience, Vol.79(2), pp.347-358
DOI: 10.1016/S0306-4522(96)00695-1
PMID: 9200720
NLM abbreviation: Neuroscience
ISSN: 0306-4522
eISSN: 1873-7544
Publisher: Elsevier Ltd
Language: English
Date published: 1997
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9983991954702771

Metrics

10 Record Views

157 Times Cited - Web of Science