Journal article
Imaging microglia in live brain slices and slice cultures
CSH protocols, Vol.2007, pp.1142-1148
10/01/2007
DOI: 10.1101/pdb.prot4852
PMID: 21356950
Abstract
INTRODUCTION This protocol describes methods for fluorescence labeling and time-lapse confocal imaging of microglia in acutely prepared tissue slices from developing and adult animals, and to slice cultures derived from early post-natal day 7 (<PND7) animals. The techniques permit real-time analyses of microglial structure and dynamic remodeling in live rodent brain tissues.
Details
- Title: Subtitle
- Imaging microglia in live brain slices and slice cultures
- Creators
- Dana KurpiusMichael E Dailey
- Resource Type
- Journal article
- Publication Details
- CSH protocols, Vol.2007, pp.1142-1148
- Publisher
- United States
- DOI
- 10.1101/pdb.prot4852
- PMID
- 21356950
- ISSN
- 1940-3402
- eISSN
- 1559-6095
- Grant note
- S10 RR017941 / NCRR NIH HHS R01 NS043468 / NINDS NIH HHS
- Language
- English
- Date published
- 10/01/2007
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984071781102771
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