Imaging of the varicella zoster virion in the viral highways: comparison with herpes simplex viruses 1 and 2, cytomegalovirus, pseudorabies virus, and human herpes viruses 6 and 7

Jorge A Padilla; Shiro Nii; Charles Grose

doi:10.1002/jmv.10330

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Imaging of the varicella zoster virion in the viral highways: comparison with herpes simplex viruses 1 and 2, cytomegalovirus, pseudorabies virus, and human herpes viruses 6 and 7

Journal article

Peer reviewed

Imaging of the varicella zoster virion in the viral highways: comparison with herpes simplex viruses 1 and 2, cytomegalovirus, pseudorabies virus, and human herpes viruses 6 and 7

Jorge A Padilla, Shiro Nii and Charles Grose

Journal of medical virology, Vol.70 Suppl 1(S1), pp.S103-S110

2003

DOI: 10.1002/jmv.10330

PMID: 12627497

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Abstract

Imaging by scanning electron microscopy (SEM) can provide insight into viral egress. At a low magnification level, varicella zoster virions (VZV) emerge from an infected cell surface in a distinctive pattern previously described as "viral highways." Viral highways consist of thousands of particles arranged in linear pathways across the syncytial surface. This egress pattern has not been described with other herpesviruses, but a systematic analysis has not been performed. Therefore, the characteristic arrangement of VZV egress was compared with that of six other members of the herpes virus family, including herpes simplex virus (HSV) types 1 and 2, human cytomegalovirus (CMV), pseudorabies virus (PRV), and human herpesvirus types 6 and 7 (HHV-6 and HHV-7). Only VZV-infected cells exhibited viral highways. Subsequent SEM examination of VZ virions at an ultra high-resolution revealed that more than 70% were aberrant. Further imaging of the other herpesviruses demonstrated that VZV structure was more closely related to PRV than HSV-1 or HSV-2. Finally, it is noted that the individual members of the herpesvirus family have distinguishable SEM profiles.

Cell Line

Herpesvirus 1, Suid - ultrastructure

Microscopy, Electron, Scanning

Herpesvirus 1, Human - ultrastructure

Humans

Herpesvirus 7, Human - ultrastructure

Herpesvirus 3, Human - ultrastructure

Animals

Herpesvirus 2, Human - ultrastructure

Swine

Cell Membrane - virology

Herpesviridae - ultrastructure

Cytomegalovirus - ultrastructure

Herpesvirus 6, Human - ultrastructure

Details

Title: Subtitle: Imaging of the varicella zoster virion in the viral highways: comparison with herpes simplex viruses 1 and 2, cytomegalovirus, pseudorabies virus, and human herpes viruses 6 and 7
Creators: Jorge A Padilla - Departments of Microbiology and Pediatrics, University of Iowa, 200 Hawkins Drive, Iowa City, IA 52242, USA
Shiro Nii
Charles Grose
Resource Type: Journal article
Publication Details: Journal of medical virology, Vol.70 Suppl 1(S1), pp.S103-S110
DOI: 10.1002/jmv.10330
PMID: 12627497
ISSN: 0146-6615
eISSN: 1096-9071
Grant note: AI 22795 / NIAID NIH HHS AI 36884 / NIAID NIH HHS
Language: English
Date published: 2003
Academic Unit: Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093499702771

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