Journal article
Imaging zinc in brain slices
CSH protocols, Vol.2007, p.pdb.prot4854
10/01/2007
DOI: 10.1101/pdb.prot4854
PMID: 21356952
Abstract
INTRODUCTIONThis protocol provides a practical guide to imaging zinc within synaptic vesicles and the extracellular space of brain slices. Certain glutamatergic synaptic terminals in the brain have high concentrations of exchangeable Zn(2+) within their synaptic vesicles. Classically, these terminals have been revealed by Timm's histochemical stain. More recently, a number of fluorimetric probes have become available that allow Zn(2+) to be detected in live preparations. This protocol describes two methods, one for visualizing Zn(2+) in synaptic vesicles, and another for detecting Zn(2+) in the extracellular space. These methods are applicable to any tissue with the high levels of loosely bound Zn(2+) that are typically found in vesicles, and tissue that might have Zn(2+) associated with macromolecules in the extracellular space.
Details
- Title: Subtitle
- Imaging zinc in brain slices
- Creators
- Alan R Kay
- Resource Type
- Journal article
- Publication Details
- CSH protocols, Vol.2007, p.pdb.prot4854
- Publisher
- United States
- DOI
- 10.1101/pdb.prot4854
- PMID
- 21356952
- ISSN
- 1940-3402
- eISSN
- 1559-6095
- Language
- English
- Date published
- 10/01/2007
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9983991947102771
Metrics
16 Record Views