Journal article
Immobilization-induced activation of key proteolytic systems in skeletal muscles is prevented by a mitochondria-targeted antioxidant
Journal of applied physiology (1985), Vol.115(4), pp.529-538
08/01/2013
DOI: 10.1152/japplphysiol.00471.2013
PMID: 23766499
Abstract
Long periods of skeletal muscle disuse result in muscle fiber atrophy, and mitochondrial production of reactive oxygen species (ROS) appears to be a required signal for the increase in protein degradation that occurs during disuse muscle atrophy. The experiments detailed here demonstrate for the first time in limb muscle that the inactivity-induced increases in E3 ligase expression and autophagy biomarkers result from increases in mitochondrial ROS emission. Treatment of animals with a mitochondrial-targeted antioxidant also prevented the disuse-induced decrease in anabolic signaling (Akt/mammalian target of rapamycin signaling) that is normally associated with prolonged inactivity in skeletal muscles. Additionally, our results confirm previous findings that treatment with a mitochondrial-targeted antioxidant is sufficient to prevent casting-induced skeletal muscle atrophy, mitochondrial dysfunction, and activation of the proteases calpain and caspase-3. Collectively, these data reveal that inactivity-induced increases in mitochondrial ROS emission play a required role in activation of key proteolytic systems and the downregulation of important anabolic signaling molecules in muscle fibers exposed to prolonged inactivity.
Details
- Title: Subtitle
- Immobilization-induced activation of key proteolytic systems in skeletal muscles is prevented by a mitochondria-targeted antioxidant
- Creators
- Erin E. Talbert - University of FloridaAshley J. Smuder - University of FloridaKisuk Min - University of FloridaOh Sung Kwon - University of FloridaHazel H. Szeto - Cornell UniversityScott K. Powers - University of Florida
- Resource Type
- Journal article
- Publication Details
- Journal of applied physiology (1985), Vol.115(4), pp.529-538
- Publisher
- Amer Physiological Soc
- DOI
- 10.1152/japplphysiol.00471.2013
- PMID
- 23766499
- ISSN
- 8750-7587
- eISSN
- 1522-1601
- Number of pages
- 10
- Language
- English
- Date published
- 08/01/2013
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Health and Human Physiology; Internal Medicine
- Record Identifier
- 9984259659202771
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