Journal article
Immune cell lethality induced by streptococcal pyrogenic exotoxin A and endotoxin
Infection and immunity, Vol.60(9), pp.3747-3755
09/1992
DOI: 10.1128/IAI.60.9.3747-3755.1992
PMCID: PMC257386
PMID: 1500185
Abstract
Streptococcal pyrogenic exotoxin (SPE) A has many effects on the immune system, including immunolethality, which is characterized by a significant decrease in circulating immune cells as well as depletion of the spleen and lymph nodes prior to death of experimental animals. In this report, characterization of the mechanism of immunolethality has been undertaken. Synergistic induction of immunolethality was observed in vitro when human lymphocytes were treated with both SPE A and lipopolysaccharide (LPS). The same effect was demonstrated in the absence of a mitogenic response with the murine T-cell receptor, as well as in the absence of antigen-presenting cells and their secreted cytokines. The addition of antigen-presenting cells did not significantly affect lethality. SPE A directly interacted with LPS through interaction with ketodeoxyoctonate as demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and iodinated exotoxin overlays. This interaction was demonstrated to be important for immunolethality, since simultaneous addition of SPE A and LPS was required, whereas sequential addition of SPE A and LPS did not result in lethality. LPS appeared to be acting, in part, to enhance the cell-binding ability of SPE A, since SPE A could only be detected in A.E7 cell membrane preparations after simultaneous incubation with SPE A and LPS.
Details
- Title: Subtitle
- Immune cell lethality induced by streptococcal pyrogenic exotoxin A and endotoxin
- Creators
- B A Leonard - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455P M Schlievert - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.60(9), pp.3747-3755
- DOI
- 10.1128/IAI.60.9.3747-3755.1992
- PMID
- 1500185
- PMCID
- PMC257386
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 09/1992
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984002385002771
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