Immune response to a murine coronavirus: identification of a homing receptor-negative CD4+ T cell subset that responds to viral glycoproteins

J Mobley; G Evans; M O Dailey; S Perlman

doi:10.1016/0042-6822(92)90446-V

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Immune response to a murine coronavirus: identification of a homing receptor-negative CD4+ T cell subset that responds to viral glycoproteins

Journal article

Open access

Peer reviewed

Immune response to a murine coronavirus: identification of a homing receptor-negative CD4+ T cell subset that responds to viral glycoproteins

J Mobley, G Evans, M O Dailey and S Perlman

Virology, Vol.187(2), pp.443-452

04/1992

DOI: 10.1016/0042-6822(92)90446-V

PMID: 1347668

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Abstract

The lymphocyte proliferative response to mouse hepatitis virus, strain JHM (MHV-JHM), a well-described cause of chronic and acute neurological infections, has been studied using vaccinia virus recombinants expressing individual MHV proteins. The surface (S) and transmembrane (M) glycoproteins were the most active proteins in causing proliferation of lymphocytes isolated from immunized adult mice, whereas lymphocytes from persistently infected mice proliferated only in response to the S protein. The cells from immunized mice which proliferated most actively in response to MHV were positive for the CD4 antigen and secreted interferon-gamma. In addition, the most responsive subset of cells did not express gp90MEL-14, the lymph node-specific homing receptor. The results identify a subpopulation of CD4+ T cells that may be an important component of the cell-mediated immune response to this virus. The data also suggest that response to the M protein is important in preventing disease progression in C57BL/6 mice since cells which recognize this protein are absent from persistently infected mice.

Flow Cytometry

Viral Matrix Proteins - genetics

Oligodeoxyribonucleotides - chemistry

Viral Proteins - immunology

Molecular Sequence Data

Antigens, Viral - genetics

Murine hepatitis virus - immunology

Base Sequence

Membrane Glycoproteins - immunology

Receptors, Lymphocyte Homing - analysis

Viral Envelope Proteins - genetics

CD4-Positive T-Lymphocytes - microbiology

Capsid - genetics

Mutagenesis, Site-Directed

Lymphocyte Activation

Mice, Inbred C57BL

Antigens, Viral - immunology

Capsid - immunology

Animals

Recombinant Proteins - immunology

Viral Core Proteins - genetics

Viral Core Proteins - immunology

Viral Envelope Proteins - immunology

Mice

T-Lymphocyte Subsets - microbiology

Viral Matrix Proteins - immunology

Interferon-gamma - biosynthesis

Details

Title: Subtitle: Immune response to a murine coronavirus: identification of a homing receptor-negative CD4+ T cell subset that responds to viral glycoproteins
Creators: J Mobley - Department of Microbiology, University of Iowa, Iowa City 52242
G Evans
M O Dailey
S Perlman
Resource Type: Journal article
Publication Details: Virology, Vol.187(2), pp.443-452
DOI: 10.1016/0042-6822(92)90446-V
PMID: 1347668
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: United States
Grant note: NS24401 / NINDS NIH HHS AI22730 / NIAID NIH HHS NS01369 / NINDS NIH HHS
Language: English
Date published: 04/1992
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Pathology; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777471802771

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