Journal article
Immune response to the immunodominant epitope of mouse hepatitis virus is polyclonal, but functionally monospecific in C57BI/6 mice
Virology, Vol.255(1), pp.106-116
1999
DOI: 10.1006/viro.1998.9576
PMID: 10049826
Abstract
Mutations in an immunodominant CD8 CTL epitope (S-510-518) are selected in mice persistently infected with the neurotropic JHM strain of mouse hepatitis virus. These mutations abrogate recognition by T cells harvested from the infected CNS in direct ex vivo cytotoxicity assays. Previous reports have suggested that, in general, an oligoclonal, monospecific T cell response contributes to the selection of CTL escape mutants. Herein, we show that, in MHV-JHM-infected mice, the CD8 T cell response after intraperitoneal infection is polyclonal and diverse. This diverse response was shown to include both polyclonal and oligoclonal components. The polyclonal data were shown to fit a logarithmic distribution. With regard to specificity, we used a panel of peptide analogues of epitope S-510-518 and spleen-derived CD8 T cell lines to determine why only a subset of possible mutations was selected in persistently infected mice. At a given position in the epitope, the mutations identified in in vivo isolates were among those that resulted in the greatest loss of recognition. However, not all such mutations were selected, suggesting that additional factors must contribute to selection in vivo. By extrapolation of these results to the persistently infected CNS, they suggest that the selection of CTL escape mutants requires the presence of a monospecific T cell response but also show that this response need not be oligoclonal.
Details
- Title: Subtitle
- Immune response to the immunodominant epitope of mouse hepatitis virus is polyclonal, but functionally monospecific in C57BI/6 mice
- Creators
- Lecia PeweStanley Perlman
- Resource Type
- Journal article
- Publication Details
- Virology, Vol.255(1), pp.106-116
- DOI
- 10.1006/viro.1998.9576
- PMID
- 10049826
- NLM abbreviation
- Virology
- ISSN
- 0042-6822
- eISSN
- 1096-0341
- Language
- English
- Date published
- 1999
- Academic Unit
- Microbiology and Immunology; University of Iowa; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983777346702771
Metrics
16 Record Views