Journal article
Immunization of healthy adults with a single recombinant pneumococcal surface protein A (PspA) variant stimulates broadly cross-reactive antibodies to heterologous PspA molecules
Vaccine, Vol.18(17), pp.1743-1754
2000
DOI: 10.1016/S0264-410X(99)00530-7
PMID: 10699322
Abstract
Pneumococcal surface protein A (PspA) is a highly variable protein found on all strains of pneumococci. To be successful, a PspA-based vaccine for
S. pneumoniae must induce antibodies that are broadly cross-reactive. To address whether cross-reactive antibodies could be induced in man, we evaluated serum from adults immunized with recombinant clade 2 PspA from strain Rx1. Immunization with 5–125 μg rPspA lead to a significant increase in circulating anti-PspA antibodies, as well as antibodies reactive to heterologous rPspA molecules. Increased binding of post-immune sera to 37 pneumococcal strains expressing a variety of PspA and capsule types was observed, versus pre-immune sera. The extent of cross-clade reactivity of human anti-rPspA followed roughly the amount of sequence homology to the non-clade 2 antigens. It is hypothesized that priming of humans by natural exposure to
S. pneumoniae contributes to the breadth of the cross-reactivity of antibody to PspA.
Details
- Title: Subtitle
- Immunization of healthy adults with a single recombinant pneumococcal surface protein A (PspA) variant stimulates broadly cross-reactive antibodies to heterologous PspA molecules
- Creators
- Gary S. Nabors - Sanofi PasteurPatricia A. Braun - Sanofi PasteurDiane J. Herrmann - Sanofi PasteurMartha L. Heise - Sanofi PasteurDerek J. Pyle - Aventis Pasteur, Discovery Drive, Swiftwater, PA 18370, USAStefan Gravenstein - Eastern Virginia Medical SchoolMargot Schilling - Eastern Virginia Medical SchoolLaura M. Ferguson - Sanofi PasteurSusan K. Hollingshead - University of Alabama at BirminghamDavid E. Briles - University of Alabama at BirminghamRobert S. Becker - Aventis Pasteur, Discovery Drive, Swiftwater, PA 18370, USA
- Resource Type
- Journal article
- Publication Details
- Vaccine, Vol.18(17), pp.1743-1754
- DOI
- 10.1016/S0264-410X(99)00530-7
- PMID
- 10699322
- NLM abbreviation
- Vaccine
- ISSN
- 0264-410X
- eISSN
- 1873-2518
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 2000
- Academic Unit
- Family and Community Medicine; General Internal Medicine; Internal Medicine
- Record Identifier
- 9984297445402771
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