Journal article
Immunobiologic and biochemical properties of mutants of toxic shock syndrome toxin-1
The Journal of immunology (1950), Vol.152(1), pp.87-95
01/01/1994
DOI: 10.4049/jimmunol.152.1.87
PMID: 8254210
Abstract
Toxic shock syndrome (TSS) is a multisystem illness caused mainly by Staphylococcus aureus producing TSS toxin-1 (TSST-1). A variant of TSST-1 has been isolated from ovine mastitis S. aureus. This toxin, TSST-ovine (TSST-O) is only weakly T cell mitogenic, is nonpyrogenic, does not enhance endotoxin shock, and does not cause TSS in the miniosmotic pump model. The sequence of the ovine gene (tstO) differs from the TSST-1 gene (tstH) by 14 nucleotides that change seven amino acids in the mature protein of which two are in the C-terminal half. A gene fusion containing half of both tstH and tstO was made and cloned into S. aureus. The fusion protein contained the two C-terminal amino acid differences that are in TSST-O at residues 132 and 140. The fusion protein was not T cell mitogenic and did not elicit TSS in two rabbit models. Additional experiments used mutagenesis to change the lysine residue at position 132 of TSST-O to glutamate (TSST-OK132E), as exists in TSST-1, and to change the lysine residue of the human-ovine fusion at position 132 to glutamate (TSST-11140T). Both mutants were pyrogenic, enhanced endotoxin shock, and caused TSS in the miniosmotic pump model. However, the proteins were only partially T cell mitogenic. The restoration of lethality of TSST-O and the human-ovine fusion by changing the lysine to glutamate, as exists in TSST-1, indicates that residue 132 is important in lethality. The failure to regenerate complete T cell mitogenicity of the same mutants indicates that residues 132 and 140 are important for that activity.
Details
- Title: Subtitle
- Immunobiologic and biochemical properties of mutants of toxic shock syndrome toxin-1
- Creators
- Debra L Murray - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455G Sridhar PrasadCathleen A EarhartBettina A B LeonardBarry N KreiswirthRichard P NovickDouglas H OhlendorfPatrick M Schlievert
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.152(1), pp.87-95
- Publisher
- United States
- DOI
- 10.4049/jimmunol.152.1.87
- PMID
- 8254210
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Grant note
- AI-22159 / NIAID NIH HHS
- Language
- English
- Date published
- 01/01/1994
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001227702771
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