Immunocapture diagnostic assays for malaria using Plasmodium lactate dehydrogenase (pLDH)

R Piper; J Lebras; L Wentworth; A Hunt-Cooke; S Houzé; P Chiodini; M Makler

doi:10.4269/ajtmh.1999.60.109

Back

Immunocapture diagnostic assays for malaria using Plasmodium lactate dehydrogenase (pLDH)

Journal article

Peer reviewed

Immunocapture diagnostic assays for malaria using Plasmodium lactate dehydrogenase (pLDH)

R Piper, J Lebras, L Wentworth, A Hunt-Cooke, S Houzé, P Chiodini and M Makler

The American journal of tropical medicine and hygiene, Vol.60(1), pp.109-118

01/1999

DOI: 10.4269/ajtmh.1999.60.109

PMID: 9988333

View Online

Abstract

We have developed two diagnostic assays based on the specific detection of Plasmodium lactate dehydrogenase (pLDH) activity. These assays exploit a panel of monoclonal antibodies that capture the parasite enzyme and allow for the quantitation and speciation of human malaria infections. An immunocapture pLDH activity assay (ICpLDH) allows for the rapid purification and measurement of pLDH from infected blood using the NAD analog APAD, which reacts specifically with Plasmodium LDH isoforms. An immunochromatographic test (the OptiMAL assay) was also formatted and allowed the detection of parasite infections of approximately 200 parasites/microl of blood. By using a combination of antibodies, both tests can not only detect but differentiate between P. falciparum and non-P. falciparum malaria. Both assays show a sensitivity comparable with other commercial nonmicroscopic tests; importantly, we found very few instances of false-positive samples, especially with samples from patients recently cleared of malaria infection. Furthermore, we find that when one uses the quantitative ICpLDH assay, the levels of pLDH activity closely mirror the levels of parasitemia in both initial diagnosis and while following patient therapy. We conclude that diagnostic tests based on the detection of pLDH are both sensitive and practical for the detection, speciation, and quantitation of all human Plasmodium infections and can also be used to indicate drug-resistant infections.

Animals

Antibodies, Monoclonal

Antibody Specificity

Chromatography

Diagnosis, Differential

False Positive Reactions

Humans

Immunoenzyme Techniques

L-Lactate Dehydrogenase - blood

L-Lactate Dehydrogenase - immunology

Malaria, Falciparum - diagnosis

Malaria, Falciparum - drug therapy

Malaria, Vivax - diagnosis

Malaria, Vivax - drug therapy

Parasitemia - diagnosis

Parasitemia - drug therapy

Plasmodium - enzymology

Plasmodium - growth & development

Details

Title: Subtitle: Immunocapture diagnostic assays for malaria using Plasmodium lactate dehydrogenase (pLDH)
Creators: R Piper - Flow, Inc., Portland, Oregon 97210, USA.
J Lebras
L Wentworth
A Hunt-Cooke
S Houzé
P Chiodini
M Makler
Resource Type: Journal article
Publication Details: The American journal of tropical medicine and hygiene, Vol.60(1), pp.109-118
DOI: 10.4269/ajtmh.1999.60.109
PMID: 9988333
ISSN: 0002-9637
eISSN: 1476-1645
Language: English
Date published: 01/1999
Academic Unit: Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
Record Identifier: 9984297594902771

Metrics

6 Record Views

177 Times Cited - Web of Science

See more details